中国小型猪细菌分离鉴定及药敏试验

Identification and susceptibility test of bacteria isolated from miniature pigs in China

目的对小型猪携带的细菌种群分布情况及耐药性进行调查分析。方法对小型猪采样进行细菌分离培养、生化鉴定和细菌16SrRNA基因PCR扩增、测序分析,并进行药敏试验。结果从25只小型猪中共分离到45株细菌。结果显示,小型猪携带的细菌种群主要有弯曲菌属(空肠弯曲菌)、螺杆菌属(幽门螺杆菌)、克雷伯菌属(肺炎克雷伯氏菌);肠杆菌属(阴沟肠杆菌);埃希菌属(大肠埃希菌、弗格森埃希菌);假单胞菌属(铜绿假单胞菌);窄食单胞菌属(嗜麦芽窄食单胞菌);葡萄球菌属(金黄色葡萄球菌、溶血葡萄球菌、模仿葡萄球菌);链球菌属(肺炎链球菌、猪链球菌、前庭链球菌、缓症链球菌、麻疹孪生球菌、绿色气球菌);肠球菌属(粪肠球菌);芽孢杆菌属(枯草芽孢杆菌、地衣芽孢杆菌、蜂房芽孢杆菌、巨大芽孢杆菌)。这些菌株对氨曲南、头孢噻吩等药物敏感,而对痢特灵等药物不敏感。结论小型猪携带的细菌种群具有多样性,研究结果为我国小型猪细菌学检测以及质量标准的制定提供了科学依据。

To investigate the prevalence and the antibiotic resistance of bacteria isolated from 25 miniature pigs.45 bacterial strains were isolated,which were identified by biochemical assays,amplification of 16S rRNA genes by PCR and sequence analysis,and were evaluated for resistance to 30 antibiotics.The identification results showed that these bacteria belonged to Campylobacter（Campylobacter jejuni）,Helicobacterium（Helicobacter pylori）,Klebsiella（Klebsiella pneumoniae）,Escherichia（Escherichia coli,Escherichia fergusonii）,Pseudomonas（Pseudomonas aeruginosa）,Stenotrophomonas（Stenotrophomonas maltophilia）,Staphylococcus（Staphylococcus aureus,Staphylococcus haemolyticus,Staphylococcus simulans）,Streptococcus（Streptococcus pneumoniae,Streptococcus suis,Streptococcus vestibularis,Streptococcus mitis,Gemella measles,Aerococcus viridans）and Bacillus（Bacillus subtilis,Bacillus licheniformis,Bacillus alvei,Bacterium megaterium）.These bacteria were all susceptible to aztreonam and cephalothin.However,the resistence to furazolidone was found.Microbial population carried by miniature pigs in China had characters of diversity.Results of this study provided scientifical accordance for the microorganism monitoring of miniature pigs in China.