EphA3受体截短突变体variant b在前列腺癌细胞中的分泌表达

Expression and Secretion Characteristic of EphA3 Variant b in Prostate Cancer Cell Lines

目的:验证EphA3受体截短突变体variant b是否是一种分泌蛋白,并探索其内源分泌的特点,在细胞水平看其是否有作为前列腺癌血清标志物的潜质。方法:构建EphA3 variant b真核表达载体,应用标签抗体、特异性抗体和Western印迹检测前列腺癌细胞培养液中EphA3 variant b的表达,用RT-PCR方法分析EphA3 variant b在8种细胞系中的表达谱和对雄激素刺激的应答。结果:验证了EphA3 variant b是一种分泌蛋白,在雄激素受体阳性的前列腺癌细胞系中特异性表达,雄激素以剂量依赖方式诱导EphA3 variant b表达。结论:EphA3 variant b是一种分泌蛋白,其表达与雄激素受体信号通路相关,有作为前列腺癌血清标志物的潜质。

Objective：To validate the truncated mutant of EphA3 receptor（EphA3 variant b）a secretary protein and to explore its endogenous secretion character,as well as to test if it can be used as a serum biomarker for prostate cancer on cellular level.Methods：By constructing eukaryotic expression vector and Western blotting with anti-His tag antibody as well as specific antibody,EphA3 variant b was tested if it can be expressed in cell culture fluid of prostate cancer cell lines.Then the expression spectrum of EphA3 variant b in eight cell lines as well as its response to androgen stimulus in LNCaP was analyzed by RT-PCR.Results：EphA3 variant b is a kind of secretary protein,which is specifically expressed in AR＋prostate cancer cell lines and dose-dependently induced by androgen.Conclusion：EphA3 variant b is a kind of secretary protein,which is androgen-receptor signal pathway associated and has its potential as a serum biomarker for prostate cancer.