摘要
目的研究足月妊娠子宫平滑肌与蜕膜组织中前列腺素F2α(Prostaglandin F2α,PG F2α)的浓度、子宫平滑肌中PGF2α受体(PG F2α recepor,PTGFR)蛋白的表达与缩宫素引产成功率的关系。方法选择缩宫素引产成功与缩宫素引产失败的孕妇,于剖宫产术中取子宫平滑肌及子宫蜕膜组织。分别行ELISA法检测组织匀浆中PGF2α的浓度,Western blot检测子宫平滑肌中PTGFR蛋白的表达。结果ELISA法证明缩宫素引产成功组子宫平滑肌、子宫蜕膜组织中PGF2α浓度显著高于缩宫素引产失败组(P<0.01);Western blot检测证明缩宫素引产成功组子宫平滑肌组织中PTGFR蛋白的表达也明显高于缩宫素引产失败组(P<0.01)。结论子宫平滑肌与蜕膜组织中PGF2α浓度、子宫平滑肌组织中PTGFR蛋白的表达量与缩宫素引产成功率关系密切。
Objective To investigate the relationship of prostaglandin F2α（PGF2α） expression in human myometrium and decidua and PGF2α receptor（PTGFR） expression in human myometrium with the success rate of labor induction with oxytocin during full-term pregnancy.Methods The cases were divided into two groups：one with success in induction of labor（n=34） by oxytocin and the other with failure of induced labor by oxytocin（n=31）.The maternal myometrial and decidual samples were isolated from uterine in cesarean section.The concentration of PGF2α in tissues was examined by enzyme-linked immunosorbent assay（ELISA）.The expression of PTGFR protein in myometrial samples was examined by Western blot.Results ELISA indicated that the concentration of PGF2α in myometrium and decidua was significantly higher in the successful labor group than in the labor failing group.Western blot indicated that the expression level of PTGFR protein in myometrium was significantly higher in the successful labor group than in the labor failing group.Conclusion The PGF2α expression in human myometrium and decidua and PTGFR expression are positively associated with the success rate of labor induced by oxytocin.
出处
《解剖科学进展》
CAS
2010年第1期45-47,50,共4页
Progress of Anatomical Sciences
基金
南京医科大学科技发展重点基金资助项目(No.2005NYDZD20)
