胎肝基质细胞强化骨髓移植治疗急性放射病

Therapy for acute radiation disease with bone marrow transplantation enhanced by fetal liver stromal cells in mice.

目的为进一步提高骨髓移植效果。方法以昆明种小鼠急性放射病为模型，进行了胎肝基质细胞强化同种异基因骨髓移植，观察了小鼠造血重建、急性移植物抗宿主病（ＧＶＨＤ）及小鼠存活率。结果胎肝基质细胞移植可强化骨髓移植效果，与单纯骨髓移植组比较，于照射后第１１天小鼠外周血白细胞、骨髓有核细胞计数，ＣＦＵ－Ｅ、ＣＦＵ－ＧＭ、ＣＦＵ－Ｆ、ＣＦＵ－Ｓ回升较快，ＣＦＵ－Ｆ已达正常，于照射后第１７天造血得以重建；ＧＶＨＤ较轻，存活率显著提高，达６０％。结论胎肝基质细胞改善了造血微环境，可能既改善了“龛位”，又增加了“龛位”使干细胞更多的植入，促进了造血重建，提高了骨髓移植效果，是一种“种子与土壤”并重的移植新方法。

Objective\ To enhance the grafting efficiency of bone marrow transplantation (BMT). Method\ The survival rate of mice,hematopoietic reconstitution,and reduction of acute graft versus host disease (GVHD) were studied in an acute radiation disease model of Kunming mice by a new method of BMT enhanced by addition of fetal liver stromal cells (BMT FLSt).Results\ WBC and nucleated cell counts and indexes of restored to normal CFU E,CFU GM,CFU S,especially CFU F,in BMT FLSt group were significantly higher than those in the group with BMT alone 11 days after irradiation.And all those indxes in BMT FLSt group returned to normal 17 days after irradiation.The survival rate of mice in BMT FLSt group (60%) was also significantly higher than that in the group with BMT alone (30%),and the degree of GVHD in BMT FLSt group was slighter than that in the group with BMT alone. Conclusion\ These data suggested that hematopoietic microenvironment could be improved,and 'niches' could be both improved and increased by fetal liver stromal cell transplantation; so,fetal liver stromal cells could prompt hematopoietic reconstitution and enhance engrafting efficiency.