利用肺癌特异性结合多肽ZS-6筛选肺癌相关标志物

Screening of tumor mark for the lung cancer by using peptide ZS-6

目的利用本实验室已获得的与肺癌特异性结合的十二肽ZS-6从人类肺癌cDNA文库中筛选出肺癌相关标志物,为肺癌的临床早期诊断和靶向治疗奠定基础。方法以生物素标记的十二肽ZS-6为探针,筛选人类肺癌cDNA噬菌体展示文库,经过四轮减性筛选后,挑取与ZS-6特异性结合的噬菌体单克隆、扩增、纯化,再通过PCR技术扩增、产物纯化后,进行DNA测序,测序结果经过生物信息学分析。结果筛选出18个与ZS-6特异性结合的噬菌体克隆,测序结果显示其中1个为未知功能的新基因,其余的均已确定为肿瘤相关基因。结论从人类肺癌cDNA噬菌体展示文库中筛选出潜在的肺癌肿瘤标志物,为肺癌的早期诊治奠定基础。

Aim To select a potential biomarker for early lung cancer diagnosis and targeted therapy by using the cancer specific bounded peptide ZS-6 which had already been obtained from the laboratory. Methods The peptide ZS-6 marked by biotin was used as a probe to pan out the human lung cancer cDNA phage display library,after 4 rounds of subtraction panning, the specific binding clones of ZS-6 were identified. After amplification and purification,then those DNA sequences were identified and analyzed with bioinformat-ics. Results 18 phage clones were identified to the specific peptide ZS-6 and the DNA sequence showed one of them was an unknown new gene while the others were known tumor related genes. Conclusion A tumor biomarker selected from human lung cancer cDNA library provides a potential tool for early lung cancer diagnosis and therapy.