异丙嗪对异氟烷小鼠镇痛、催眠、遗忘作用和治疗指数的影响

The effects of promethazine on the analgesia,hypnosis,amnesia and therapeutic index of isoflurane treated mice

目的观察异丙嗪对异氟烷镇痛、催眠、遗忘作用和治疗指数的影响。方法用热板法和扭体法观察异丙嗪对异氟烷镇痛作用的影响,翻正反射法观察异丙嗪对异氟烷小鼠睡眠时间的影响,Morris水迷宫法观察异丙嗪对异氟烷小鼠遗忘作用的影响,并用序贯法观察异丙嗪对异氟烷小鼠催眠ED50、LD50的影响。结果在热板法和扭体法实验中,异丙嗪增强了异氟烷的镇痛作用(P<0.05或P<0.01);在翻正反射实验中,异丙嗪可延长异氟烷小鼠的睡眠时间(P<0.01);在水迷宫实验中,异丙嗪组和异氟烷组的平均逃避潜伏期均少于两药合用组(P<0.05或P<0.01),第3象限停留时间均多于两药合用组(P<0.01或P<0.05),异氟烷组原平台穿越次数多于合用组(P<0.05);异丙嗪可降低异氟烷小鼠的催眠ED50(P<0.01),而对其LD50无影响(P>0.05)。结论异丙嗪可以增强异氟烷的镇痛、催眠和遗忘作用,并可以提高异氟烷的治疗指数。

Aim To observe the effects of promethazine on the analgesia,hypnosis,amnesia and therapeutic index of isoflurane. Methods The experiments were designed to study promethazine on the analgesic effect of isoflurane by hot-plate test and writhing test,and to study the effect of promethazine on the sleeping time of isoflurane by the method of righting reflex,and the amnesia of isoflurane by Morris water maze,and the ED50, LD50 by sequential method in mice. Results The result of hot-plate test and writhing test indicated that promethazine could enhance the analgesic effect of isoflurane（P〈0.05 or P〈0.01）; through the experiment of righting reflex,sleeping time of isoflurane in mice was extended by promethazine （P〈0.01）; in Morris water maze experiment,the average latency in the combination of promethazine and isoflurane was longer than that of the promethazine group or isoflurane group（P〈0.05 or P〈0.01）,while aiming to the residence time,the combination of the two was shorter than that in the third quadrant （P〈0.01 or P〈0.05）,the TCPP of the group of isoflurance was more than that of the combination group; promethazine could decrease the ED50 of isoflurance（P〈0.01）,but it did not obviously affect its LD50（P〈0.05）. Conclusion Promethazine can not only reinforce the effect of isoflurance on analgesia,hypnosis and amnesia,but also boost the therapeutic index of isoflurance.