食管鳞状细胞癌患者外周血和肿瘤组织中ERCC1及ERCC2表达的相关性研究

Relationship between the expressions of ERCC1 and ERCC2 in peripheral venous blood and cancer tissues of esophageal squamous cell carcinoma patients

目的:探讨检测食管鳞状细胞癌患者外周血替代肿瘤组织中切除修复交叉互补基因1(excision repair cross-comple-menting 1,ERCC1)及ERCC2表达的可行性。方法:收集39例食管癌患者的术前外周血、肿瘤组织及癌旁正常组织,应用RT-PCR法检测ERCC1和ERCC2 mRNA的表达,应用ELISA法检测血清蛋白中ERCC1和ERCC2的含量,以10名健康志愿者外周血作为对照。结果:ERCC1和ERCC2 mRNA以及蛋白在健康志愿者外周血中的表达高于食管癌患者外周血(P<0.05);食管癌患者外周血中ERCC1和ERCC2 mRNA的表达水平与其在肿瘤组织中的表达水平呈明显的正相关关系(P<0.01)。结论:食管鳞状细胞癌患者外周血中ERCC1和ERCC2 mRNA的表达水平能在一定程度上间接反映其在肿瘤组织中的表达水平。

Objective：To investigate the feasibility of detecting excision repair cross-complementing 1（ERCC1）and ERCC2 in peripheral venous blood instead of cancer tissues from esophageal squamous cell carcinoma patients.Methods：The expressions of ERCC1 and ERCC2 mRNA were detected by using RT-PCR in 39 cases of peripheral venous blood samples,esophageal squamous cell carcinoma tissues,and adjacent normal tissues.ELISA was used to determine the levels of ERCC1 and ERCC2 proteins in serum.The periphe-ral blood from 10 healthy volunteers was used as control.Results：Expression levels of ERCC1 and ERCC2 mRNA and protein were significantly higher in peripheral blood from healthy control than those in esophageal carcinoma patients（P0.05）.There was a positive correlation between the expression of ERCC1 and ERCC2 mRNA in peripheral blood and cancer tissues（P0.01）.Conclusion：The expression levels of ERCC1 and ERCC2 mRNA in peripheral blood can indirectly reflect their expression levels in human esophageal squamous cell carcinoma tissues.