马鞭草抗早孕作用机理的初步研究

Mechanism of Contragestational Effect of Verbena Officinalis Extract

为探讨马鞭草抗早孕的细胞学作用机理，以正常妊娠６～８周早孕绒毛组织体外培养为实验手段，观察了马鞭草乙醇提取液对绒毛滋养层细胞光镜、电镜、免疫组织化学等形态学变化及对绒毛膜促性腺激素（ＨＣＧ）分泌功能的影响。结果表明：２５ｇ／Ｌ马鞭草提取液能明显抑制细胞生长及激素分泌；加药后４８小时超微结构显示，微绒毛明显减少，内质网高度扩张，线粒体有髓样改变，染色质固缩、并聚集于核膜下；ＨＣＧ分泌量４８小时后仅为对照组的１／４。免疫组织化学反应也显示，在滋养层细胞内ＨＣＧ及ＳＤＨ明显减弱。一定浓度的马鞭草提取液以剂量依赖方式直接损伤滋养层细胞，抑制ＨＣＧ的合成和分泌。

To study the mechanism of contragestational effect of alcohol extract of Verbena officinalis(VO), we observed both the changes of cell morphology, which is observed under phase-contrast microscope and electron microscope or by histochemical study, and the amount of HCG secreted after treatment with VO in 6-8 weeks cultured normal placental tissues in vitro. Our results showed that VO at 25, 50 mg/ml inhibited not only cell growth but also HCG secretion significantly. The histochemical study showed that the amount of HCG secreted and the activity of succinate dehydrogenase (SDH) of trophoblast cells in the VO treated groups were much weaker than that in the control groups. Pathological changes including decreased microvilli, disintegrated cell membrane, enlarged endoplasmic reticula cisternae, myelinlikely changed mitochondria and condensed chromatins arranged below nuclear membrane were also shown in the trophoblast cell in VO treated groups. Conclusively, these data in present study suggested that VO of certain concentrations directly damaged trophoblast cells and inhibited HCG secretion, so they provided some theoretical evidence for the clinical contragestational application of VO.