小鼠睾丸组织培养时间及温度对精子生成影响的观察

Effects of Tissue Culturing Time and Temperature on Spermatogenesis in Mouse Testis

为建立小鼠睾丸组织短期培养方法，对成年小鼠睾丸组织进行培养。结果显示：在３４℃条件下培养２４小时，小鼠睾丸组织各级生精细胞和间质细胞发育正常。培养４８小时，曲精小管基底部细胞发生增殖。培养７２小时，曲精小管基底层增殖的细胞向管腔面迁移。在３７℃条件下，生精上皮细胞发生脱落和崩解。结果提示，本研究建立的小鼠睾丸组织短期培养方法能有效减少曲精小管微环境的改变。

Using the method of tissue culture mouse testes were cultured. After 24 hours of culture at 34℃, development of spermatocytes and Leydig cells were all normal. After 48 hours, spermatocytes near the basement membrane of seminiferous tubules were proliferated. After 72 hours, proliferated spermatocytes moved towards the lumen of seminiferous tubules. When cultured at 37℃, spermatocyte became necrosed and exfoliated. The results indicated that short time tissue culture could effectively reduce the change of micro environment of seminiferous tubules. The resuet may be useful in long time testis tissue culture.