四种纳米粒径钛片生物相容性检测

Biocompatibility of Titanium Films with Different Nano-size

目的研究4种纳米粒径钛片的生物相容性。方法分别在常温、100℃、250℃和380℃,采用直流磁控溅射法,形成4种纳米粒径表面的钛片。在24孔培养板上,将SD(Sprague-Dawley)乳鼠第3代成骨细胞接种于4种纳米粒径的钛片和未处理的钛片表面,分别为常温组、100℃组、250℃组、380℃组、非处理组;另设1个空白对照组,细胞培养板内不放入钛片。四甲基偶氮唑盐比色法测定6组细胞的增殖情况。结果培养后第1、4、7天,250℃组和380℃组材料表面细胞持续增殖,其中250℃组最高;培养后第7天,4个温度处理组细胞光密度值与非处理组的差异均有统计学意义(P<0.05)。结论纳米化纯钛材料表面可促进成骨细胞的增殖。

Objective To explore the biocompatibility of implant materials after surface modification,and to test cell proliferation on the nano-scale titanium films of titanium surface. Methods According to different sputtering temperatures,four different nano-granule titanium films were prepared by direct magnetron sputtering. Rat osteoblasts were seeded on the surface of different nano-granule titanium film samples and non-treated Ti sample （control group）. Cell proliferation on titanium surface was evaluated by using [3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H-tetrazoliumbromide,MTT] colorimetry. Results Cell proliferation test results in titanium surface showed that with the incubation time from1 d,4 d to 7 d,250 ℃ and 380 ℃,in the experimental group the cells showed continuous proliferation,and reached the highest value at 250 ℃. Significant differences were observed between those from experimental groups and control. （P〈0.05）. Conclusion Nanosizing titanium surface could significantlyimprove osteoblast proliferation.