非小细胞肺癌组织中MMP-12的表达及临床意义

Clinical significance of MMP-12 expression in non-small cell lung cancer:report of 38 cases

目的探讨非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中MMP-12的表达及临床意义。方法应用逆转录-聚合酶链反应(RT-PCR)检测12例Ⅰ期和26例Ⅱ、Ⅲ期非小细胞肺癌及癌旁组织中MMP-12 mRNA的表达;Western blot检测部分标本中MMP-12蛋白表达。结合临床病理资料,比较MMP-12 mRNA表达与非小细胞肺癌临床病理特征的关系。结果MMP-12 mRNA在38例癌旁组织中无表达,在12例Ⅰ期和26例Ⅱ、Ⅲ期癌组织中呈不同程度的表达,半定量均值分别为(0.551±0.140)和(1.082±0.424),其差异有统计学意义(P=0.001);MMP-12蛋白在癌旁组织中无表达,而在Ⅰ期癌组织中低表达,在Ⅱ、Ⅲ期癌组织中高表达。结论MMP-12在非小细胞肺癌组织中高表达,并与淋巴结的转移存在显著相关性,表明MMP-12基因转录水平异常在非小细胞肺癌的发生、发展中起了重要作用。

Objective To investigate the expression and clinical significance of matrix metalloproteinase-12（MMP-12） in non-small cell lung cancer（NSCLC）.Methods Reverse transcription-polymerase chain reaction（RT-PCR） was used to detect MMP-12 mRNA expressions in 38 NSCLC the tumor tissues,including 12 samples of stage Ⅰ and 26 of stage Ⅱ and Ⅲ,and the matched normal lung tissues.Western blotting was used to measure the protein expression of MMP-12 in some tissue samples.Clinicopathological data were collected,and the relationship of MMP-12 mRNA and MMP-12 protein expression were analyzed with the clinicopathological characteristics of NSCLC.Results There was no expression of MMP-12 in 38 matched normal lung tissue,and MMP-12 mRNA was expressed in the NSCLC at stage Ⅰ（0.551±0.140）,and at stage Ⅱ and Ⅲ tumor tissues（1.082±0.424） with significant difference（P=0.001）,MMP-12 protein was not found in normal lung tissues,but expressed mild in the cancer tissue at stage Ⅰ,and highly in the tissues at stage Ⅱ and Ⅲ.Conclusion MMP-12 is highly expressed in NSCLC at stage of Ⅱ and Ⅲ,and significantly correlated with lymphatic node metastasis.The abnormal expression of MMP-12 in gene transcription may play an important role in the tumorogenesis and development of NSCLC.