胃癌细胞基质金属蛋白酶-2、9的表达及对细胞迁移的影响

Expression of Matrix Metalloproteinase-2 and-9 and Effect on Invasion of Two Gastric Cancer Cell Lines

目的研究基质金属蛋白酶-2、9(matrix metalloproteinase-2、9,MMP-2、9)在胃癌细胞株HGC-27和SGC-7901的表达及对细胞迁徙转移的影响。方法采用Real-time PCR检测MMP-2、9 mRNA的含量;以ELISA检测培养上清液中MMP-2、9的相对浓度;通过明胶酶谱法测定培养上清液中的MMP-2、9比活。用Transwell小室迁徙实验、同质黏附和异质黏附实验比较两株细胞体外迁徙黏附能力。结果HGC-27中MMP-2和MMP-9 mRNA含量高于SGC-7901。HGC-27上清液MMP-2、9相对浓度均高于SGC-7901,且MMP-2比活高于SGC-7901,两株细胞MMP-9比活相近。HGC-27穿膜细胞数和异质黏附细胞数高于SGC-7901,同质黏附细胞数低于SGC-7901,差异均有统计学意义(P<0.05)。结论迁徙和异质黏附能力较强、同质黏附能力较弱的胃癌细胞HGC-27的MMP-2、9表达较强,提示MMP-2、9高表达可能促进胃癌细胞迁徙转移。

Objective To compare the expression of MMP-2 and MMP-9 and explore the effects of MMP- 2 and MMP-9 on invasion and metastasis of gastric cancer cell lines HGC27 and SGC7901. Methods The mRNA levels of MMP-2 and MMP-9 were detected using Real-time PCR. Relative concentrations of MMP-2 and MMP-9 in the culture medium were measured with ELISA. Though gelatinase zymography, specific activities of MMP-2 and MMP-9 in the culture medium were analyzed. By transwells chamber model assay, the invasion abilities of two cell lines were compared, and using homogeneous adhesion and heterogeneous adhesion experiments their adhesion ability were compared. Results The level of MMP-2 and MMP-9 mRNA in HGC-27 were higher than that in SGC-7901. The relative concentrations of MMP- 2 and MMP-9 in HGC-27 culture medium were higher than that in SC,-C-7901 culture medium. The spe- cific activity of MMP-2 in HGC-27 culture medium was stronger than that in SGC-7901 medium, meanwhile, the specific activities of MMP-9 were similar in two cell lines. For HGC-27, transmembrane cell count and heterogeneous adhesion cell count were more than the SCK2-7901, and homogeneous adhesion cell count was less than SGC-7901 （P〈0. 05）. Conclusion Compared with SGC-7901, the expressions of MMP-2 and MMP-9 of HGC-27 were higher, and the invasion and heterogeneity adhesion ability were stronger, but the homogeneous adhesion capacity was weaker. The results suggest that the expression of MMP-2 and MMP-9 may promote the invasion and metastasis of cancer cell.