宫颈癌中HPV16感染与IL-6表达的关系

Relationship between HPV16 Infection and IL-6 Expression in Cervical Carcinoma

目的探讨IL-6在宫颈病变中的表达及其与癌浸润转移的关系,探讨宫颈病变组织中HPV16DNA的表达及临床意义,探索两者之间的关系。方法采用免疫组织化学SP法检测了40例宫颈鳞状细胞癌(CSCC)、38例CINⅢ、28例CINⅠ～Ⅱ和15例正常宫颈组织中IL-6的表达;采用原位杂交法检测各组中HPV16DNA的表达。结果IL-6在正常对照组、CINⅠ～Ⅱ组、CINⅢ组和CSCⅣ组阳性率分别为46.7%(7/15)、71.4%(20/28)、81.6%(31/38)、87.5%(35/40),CSCC组的表达高于正常对照组及CIN1-2组(P<0.05),且与临床分期和病理分级无关,但与淋巴结转移有关;上述各组中HPV16DNA阳性表达率分别为6.7%(1/15)、25%(7/28)、65.8%(25/38)、80%(32/40),各组明显高于正常对照组(P<0.05);CINⅢ组明显高于CINⅠ～Ⅱ组(P<0.05);HPV16DNA的表达与CSCC临床分期、病理分级及淋巴结转移无关;IL-6和HPV16DNA表达呈正相关(rs=0.756,P<0.05)。结论IL-6在CSCC中的高表达与宫颈癌的侵袭性行为有关,可作为判断早期宫颈癌预后的指标;IL-6和HPV16感染呈正相关,二者可能协同作用导致宫颈癌的恶性发展。

Objective To study the expression of interleukin-6 （IL-6） in cervical carcinoma and its relationship with invasion and metastasis and explore the expression of HPV16 DNA in cervical disease and the correlation of IL-6 with HPV16 infection. Methods The expression of IL-6 in 40 cases of CSCC, 38 cases of CIN3, 28 cases of CIN Ⅰ-Ⅱ, 15 cases of normal cervical epithelium was detected by immunohistochemical SP method, and the expression of HPV16 DNA in the four groups were detected by in situ hybridization. Results The positive rate of IL-6 in normal cervical epithelium, CIN Ⅰ-Ⅱ, CINⅢand CSCC was 46. 7% （7/15）, 71.4% （20/28）, 81.6% （31/38）, 87. 5% （35/40） respectively. The positive rate of IL-6 in CSCC was significantly higher than in normal controls and CIN Ⅰ-Ⅱ （P（0. 05）. The expression of IL-6 was not associated with clinic stage and histological grade, but to metastasis of lymph node. The positive rate of HPV16 DNA in normal cervical epithelium, CIN Ⅰ-Ⅱ , CIN Ⅲ and CSCC was 6. 7%（1/15）, 25 % （7/28） , 65. 8% （25/38） and 80% （32/40）, respectively, and that was significantly higher in other three group than in normal controls. There was significant difference between CIN Ⅲand CIN Ⅰ-Ⅱ. The expression of HPV16 DNA was not associated with clinic stage, histological grade and metastasis of lymph node. The expression of IL-6 was positively related to HPV16 DNA （rs = 0. 756, P〈0. 05）. Conclusion The high expression of IL-6 in CSCC might be correlated with invasive behavior of cervical carcinoma, and IL-6 might become a useful prognostic indicators for early cervical carcinoma. IL-6 was positively related to HPV16 infection in CSCC, and their synergistic reaction might lead to the development of cervical carcinoma.