急性髓系白血病骨髓单个核细胞糖基化磷脂酰肌醇特异性磷脂酶D mRNA的表达与意义

Expression and Activity of Glycosylphosphatidilinoditol-Specific Phospholipase D mRNA in Bone Marrow Mononuclear Cells Isolated from Patient with Acute Myeloid Leukemia and Their Significance

本研究探讨糖基化磷脂酰肌醇特异性磷脂酶D(glycosylphosphatidilinoditol-specific phospholipase D,GPI-PLD)在急性髓系白血病病人骨髓单个核细胞中的表达及其意义。采用半定量RT-PCR和酶转化底物的百分率的方法检测急性髓系白血病初发组、难治复发组、缓解组与正常对照组骨髓单个核细胞GPI-PLD酶活性和mRNA表达,并比较它们之间的差异。结果显示:急性髓系白血病初治组骨髓单个核细胞GPI-PLD mRNA表达和GPI-PLD活性分别为1.86±0.32、(46.96±7.15)%;缓解组分别为1.26±0.29和(33.36±5.13)%;难治复发组分别为1.79±0.19和(44.31±7.22)%;而正常对照组分别为1.27±0.23和(35.38±5.15)%。初治组和难治复发组的GPI-PLD活性与mRNA表达均明显高于正常对照组,差异具有统计学意义(p<0.01);而缓解组与正常对照组之间比较,差异无统计学意义(p>0.05)。结论:GPI-PLD活性和mRNA表达水平变化一致;初治组和难治复发组急性髓系白血病病人骨髓单个核细胞GPI-PLD活性与mRNA表达水平明显高于正常组。GPI-PLD是否在白血病的发生、发展过程中发挥一定作用,有待进一步研究。

This study was purposed to investigate the expression and significance of glycosylphosphatidilinoditolspecific phospholipase D （GPI-PLD） in bone marrow mononuclear cells （BMMNC） isolated from patients with acute myeloid leukernia（AML）, GPI-PLD activity in BMMNC isolated from 78 patients with AML and 15 normal persons was measured by using GPI-anchored placental alkaline phosphatase （PLAP） as a substrate and Triton X-114 phase partitioning. The GPI-PLD mRNA expression was measured by semi-quantitive reverse transcription-polymerase chain reaction （RT-PCR）. The results showed that the mRNA expression level and activity of GPI-PLD in BMMNC from de novo AML patients were 1.86 ± 0.32 and 46.96 ± 7.15% respectively; the mRNA expression level and activity of GPI-PLD in BMMNC from completely remission and refractory or relapsed patients were 1.26 ± 0.29, 33.36±5.13% and 1.79 ±0.19, 44.31 ±7.22%, while those in BMMNC from normal controls were 1.27±0.23, 35.38 ±5.15% respectively. The mRNA expression level and activity of GPI-PLD in de novo and refractory or relapsed patients were obviously higher than those in normal controls with significant difference （p 〈 0.01 ）, while the comparison between remitted patients and normal controls showed no statistical difference （p 〉 0.05 ）. It is concluded that the expression level of GPI-PLD mRNA coincides with GPI-PLD activity. The mRNA expression and activity of GPI-PLD in de novo and refractory or relapsed patients are obviousily higher than those in normal controls. It is worthy of further exploring whether GPI-PLD plays a certain role in process of leukemia pathogenesis.