复方浙贝颗粒联合阿霉素对K562／A02移植瘤细胞膜转运蛋白表达的影响

Effects of Compound Zhe-Bei Granule(CZBG) Combined with Doxorubicin on Expression of Membrane Transport Proteins in K562/A02 Cell Xenografts

为了观察复方浙贝颗粒(CZBG)联合阿霉素对K562/A02移植瘤细胞膜耐药相关蛋白P-gp、MRP、LRP表达的影响,将K562/A02细胞接种于BALB/c-nu裸小鼠腋前皮下以构建MDR移植瘤动物模型,给予复方浙贝颗粒灌胃联合阿霉素腹腔注射,实验结束后处死小鼠,剥离肿瘤,将瘤块组织制成切片,通过免疫组织化学结合Image ProP lus6.0免疫组织化学彩色图象分析,观察复方浙贝颗粒联合阿霉素对K562/A02移植瘤组织细胞膜P-gp、MRP、LRP表达的影响。结果表明:与阿霉素组相比,CZBG各剂量组移植瘤细胞膜所表达P-gp、MRP的积分光密度值(IOD)明显降低(p<0.05),而未见明显LRP表达。结论:CZBG能协同阿霉素抑制K562/A02移植瘤组织细胞膜P-gp、MRP表达。

This study was purposed to investigate the effects of compound Zhe-Bei granule （CZBG） combined with doxorubicin on expressions of P-gp, MRP, LRP in K562/A02 cell xenografts. Tumor xenograft model were established by injecting the multidrug resistant cell line K562/A02 in the axillary flank of BALB/c-nu-nu mice. CZBG-intragastric administration and doxorubicin-intraperitoneal injection in combination were given to the BALB/c-nu nude mice. The tumor xenografts were made into slice after the dissection, and the expression of P-gp, MRP, LRP in K562/A02 tumor xenografts in mice were investigated by immunohistochemical technique. The integral optical density （IOD） of P-gp, MRP, LRP in K562/A02 tumor xenografts were measured by Image ProPlus 6.0. The results showed that as compared with the doxorubicin alone, the combination of the doxorubicin and CZBG with high, middle and low dosage could de- crease IOD of P-gp,MRP in K562/A02 tumor xenografts with statistical significance（p 〈0.05）. The LRP expression in K562/A02 tumor xenografts was not observed in five groups. It is concluded that the combination of CZBG with doxoru- bicin decreases the expressions of P-gp,MRP in K562/A02 tumor xenografts of mice.