磁性纳米四氧化三铁对小鼠淋巴细胞及巨噬细胞功能影响的初步研究

Influence of Magnetic Fe_3O_4 Nanoparticle on Functions of Lymphocytes and Macrophages in Mice

为了探讨磁性纳米四氧化三铁颗粒(Fe3O4-MNP)对小鼠免疫系统的影响,将ICR小鼠随机分为4组,分别给予生理盐水、低剂量、中剂量和高剂量Fe3O4-MNP,在小鼠经胃灌药后2周处死,测量脾脏重量及小鼠体重并计算脾脏指数;用刀豆蛋白A(ConA)诱导的小鼠脾淋巴细胞转化试验测定脾淋巴细胞增殖活性,中性红比色法测定小鼠腹腔巨噬细胞吞噬功能。结果表明,与对照组相比,实验组的脾脏指数并没有明显差异(p>0.05)。实验组脾淋巴细胞增殖率均高于对照组,尤其在中剂量组(p<0.05),且中剂量组腹腔巨噬细胞吞噬率较对照组及其他2个实验组均有明显升高(p<0.05)。结论:合适剂量的Fe3O4-MNPs可以增强小鼠淋巴细胞免疫和巨噬细胞吞噬功能。

This study was purposed to investigate the effects of magnetic nanoparticle of Fe3O4 （ Fe3O4-MNPs） on murine immune system-1CR mice were assigned randomly into four groups which were treated with normal saline, low, middle and high dose of MNP-Fe3O4 respectively. The mice were killed after being exposed by intragastric administration for 2 weeks. The ratios of spleen weight to body weight, lymphocyte transformation rate in spleen suspension and phagocytic index of macrophage in abdominal cavity were detected. The results showed that the ratios of spleen weight to body weight in Fe3O4-MNP groups were not significantly different in comparison with the control （p 〉 0.05 ）. The lymphocyte transformation rate in spleen suspension in Fe3O4-MNP groups were all higher than that in control group（ - 0.1775 ± 0.0246 ）, especially in the middle dose group （ 0. 1833 ± 0. 0593 ） （p 〈 0.05 ）, and the phagocytic index of macrophages in abdominal cavity of middle dose group （0. 2051 ± 0. 0213 ） was higher than that of control group and other two Fe3O4 -MNP group （ low dose 0.1538 ± 0.0100, high dose 0.1511 ± 0.0184 ） （p 〈 0.05 ）. It is concluded that suitable dose of Fe304-MNP can enhance the cellular immune activity and phagocytic function of macrophages of mice.