M-FISH技术在复杂核型异常的急性白血病患者的诊断、预后分析中的应用

M-FISH Technique in Diagnosis and Prognostic Analysis for Acute Leukemia with Complex Chromosomal Aberrations——Review

M-FISH,即multi-coclour FISH和multiplex FISH(M-FISH),是分子细胞遗传学方面在过去的10年里最具有发展意义的代表技术之一。这项技术从最初使人类23对染色体上24种特异的荧光色彩以供核型分析,到现在已经产生了许多复杂的变化,也开始被各种不同专业的领域范围所应用。在白血病细胞遗传学上,M-FISH已被应用于下列染色体异常患者的检查:①可疑有未被发现的微小的染色体易位;②具有复杂异常核型的染色体异位(普通方法很难获得完全的染色体核型)。最终的检查结果对白血病患者的诊断、治疗及预后分析具有很大的指导作用。本文就M-FISH的技术原理和常用的探针、M-FISH在急性白血病的诊断、疗效评价及预后分析中的应用进行了综述。

The M-HSH includes multi-coclour HSH and multiplex HSH, it represents one of the most significant developments in molecular cytogenetics of the past decade. This technique was originally designed to generate 24 colour karyotyping in human＇s 23 pair chromosome, now the technique has many variations and has been used in different fields. In leukaemia cytogenetics, the M-HSH now is used in detection for AL patients with following chromosome abnormality： （1） harbouring minimal chromosome translocation is respected; （2） chronosome translocation with complex abmormal karyotypes exists in patients with leukemia which are difficultly detected by using conventional method. The final results detected by M-FISH have guide significance for diagnosis, therapy and prognosis of AL patients. In this asticle the technical basis with comononly used probe for M-FISH, application of M-FISH in diagnosis, evaluation of therapeutic effecacy and prognostic analysis of AL patients are sammarised.