上呼吸道淋巴瘤的病理、免疫表型及其与EB病毒相关性的研究

Investigation of the clinicopathologic features, immunophenotype and EpsteinBarr virus infection of the upper respiratory tract lymphomas in patients

目的研究中国北方人上呼吸道淋巴瘤的临床病理学、免疫表型及与ＥＢ病毒的关系。方法应用单克隆抗体ＵＣＨＬ１、Ｌ２６、４ＫＢ５及ＣＳ１４进行免疫组化染色，ＥＢ病毒寡核苷酸探针（ＥＢＥＲ１／２）原位杂交，观察１１２例上呼吸道淋巴瘤的免疫表型及ＥＢ病毒感染情况。结果１１２例上呼吸道淋巴瘤病例中７７例为Ｔ细胞淋巴瘤（６８８％）。３５例为Ｂ细胞淋巴瘤（３１．３％）。ＥＢＶＥＢＥＲ１／２原位杂交１０１例中５４例阳性，各例中阳性细胞占肿瘤细胞的１５０％～８５０％，阳性病例中Ｔ细胞淋巴瘤４８例（８８９％），Ｂ细胞淋巴瘤６例（１１１％）。结论中国北方人上呼吸道淋巴瘤以Ｔ细胞淋巴瘤为多见，且与ＥＢ病毒有较高的相关性。

Objective To study the clinicopathologic features, immunophenotype and EpsteinBarr virus (EBV) infection of the upper respiratory tract lymphomas in patients from north China. Methods 112 cases of upper respiratory tract lymphomas in patients from north China were investigated. HE stain for histologic features. Immunohistochemistry studies were performed using monoclonal antibodies, UCHL1 for Tlymphocyte, L26 and 4KB5 for B lymphocyte, CS14 for latent membrane protein1 (LMP1). In situ hybridization for EBV encoded small nuclear RNA (EBER 1/2, Y017, DAKO). Results 77/112 cases (68.75%) were Tcell lymphomas, 35 cases (31.25%) were B cell lymphomas. EBVEBER 1/2 in situ hybridization positive in 54/101 (53.46%) cases, including 48 Tcell lymphoma cases and 6 Bcell lymphoma cases. Conclusion Most upper respiratory tract lymphoma cases in patients from north China are Tcell lymphomas and are strongly associated with EBV.