CD44V6的表达对大肠癌细胞骨架的影响

Expression of CD44V6 and its effects on cytoskeleton in human colorectal carcinoma cell lines

目的研究ＣＤ４４Ｖ６在大肠癌细胞系ＨＴ２９、ＬｏＶｏ细胞中的表达及其对细胞骨架的影响，探讨ＣＤ４４Ｖ６影响肿瘤转移的作用机理。方法应用原位分子杂交、免疫组化、免疫荧光定量分析的方法研究ＣＤ４４Ｖ６在人大肠癌细胞中的表达，应用免疫荧光及共聚焦三维图像重构分析的方法，研究ＣＤ４４Ｖ６对大肠癌细胞的细胞骨架的影响。结果ＣＤ４４Ｖ６在ＨＴ２９、ＬｏＶｏ细胞中均有表达，不管在ｍＲＮＡ水平还是在蛋白质水平，高转移能力的ＬｏＶｏ细胞表达ＣＤ４４Ｖ６均高于低转移能力的ＨＴ２９细胞。将ＨＴ２９、ＬｏＶｏ细胞用ＣＤ４４Ｖ６单抗封闭则发现ＣＤ４４Ｖ６能使ＨＴ２９、ＬｏＶｏ细胞的细胞骨架发生改变。结论ＣＤ４４Ｖ６的表达与大肠癌细胞的转移潜能有关。ＣＤ４４Ｖ６可能通过影响大肠癌细胞骨架的构像和分布，从而影响大肠癌细胞的运动能力。

Objective To observe the expression of CD44V6 and its effects on cytoskeleton in human colorectal carcinoma(HCC) HT29 and LoVo cell lines. Methods Expression of CD44V6 variant in HCC HT29 and LoVo cell lines was investigated by in situ hybridization, immunohistochemistry and quantitative immunofluorescence technique. The effects of CD44V6 variants on cytoskeleton was studied by immunofluorescence and confocal 3 dimension reconstruction technique. Results CD44V6 was expressed at the mRNA and protein levels in both HT29 and LoVo cell lines, but the expression was higher in LoVo cells than in HT29 cells. When the HT29 cells and LoVo cells were blocked by monoclonal antibodies, it was found that changes developed in the cytoskeleton of these cells. Conclusions The expression of CD44V6 is related to the metastatic potentiality of human colorectal carcinoma cells. CD44V6 may affect the distribution, polymerization and depolymerization of actin of HCC cells and promote HCC cell metastasis.