期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

HGFs联合对体外人脐血造血干细胞及SCID小鼠体内扩增作用的研究 被引量：4

下载PDF
导出
摘要 本实验应用外源性重组人多种造血生长因子(rhHGFs)处理人HSC/SCID小鼠模型,以体外半固体培养法及FCAS检测CFU-GM、CFU-GEMM及CD34^+细胞。从而评价HGFs对受鼠体内人造血干细胞的扩增作用。结果:人脐血及骨髓MNC经孵育24小时后CD34^+细胞含量,CFU-GM、CFU-GEMM产率皆得到明显扩增(P<0.01),且脐血组优于骨髓组(P<0.01)。输脐血MNC+HGFs组小鼠骨髓MNC中人CD34^+细胞含量、CFU-GM及CFU-GEMM产率皆明显高于单输脐血MNc组(P<0.01)及输骨髓MNC+HGFs组(P<0.01),表明脐血造血干细胞对HGFs作用更敏感,扩增潜能更强。
作者 黄绍良 周敦华 李树浓 候玲玉
机构地区 中山医科大学附属医院
出处 《临床儿科杂志》 CAS CSCD 北大核心 1998年第4期259-259,共1页 Journal of Clinical Pediatrics
基金 国家自然科学基金 美国CMB及广东省卫生厅重点课题资助
关键词 造血生长因子 脐血造血干细胞 体内扩增
分类号 R457.9 [医药卫生—治疗学]
  • 相关文献

参考文献5

  • 1何志巍,李树浓,黄绍良,赵波.人脐血细胞在SCID小鼠体内生长特性的研究[J].中国实验血液学杂志,1995,3(2):160-164. 被引量：1
  • 2黄绍良,侯玲玉,梅少芬.38例脐血粒系祖细胞体外培养的观察[J].临床儿科杂志,1991,9(4):226-227. 被引量：6
  • 3Namirawa R, Weibaecher KN, Kuneshima H, et al. Enhancement of human hematopoiesis in the SCIDhu mouse. J Exp Med, 1990,172:1055.
  • 4Almeida-porada CD, Hoffman R, Ascensao, et al. Enhancement of human hematopoietic stem cells engraftment and expression by human stroma in human/sheep chimeras. Exp Hematol, 1994,22(8):739.
  • 5Lapidot T, Pflumio F, Doedens M, et al. Cytokine stimulation of multilineags hematopoiesis from immature human cells engrafted in SCID mice. Science, 1992,255:1137.

二级参考文献14

  • 1黄绍良,李文益,侯玲玉,杜宏微,李树浓.人胎盘血粒单系和红系祖细胞体外培养观察[J].中国实用儿科杂志,1994,9(4):225-226. 被引量：4
  • 2Broxmeyer HE,Hangoc G,Cooper S,et al.Growth characteristics and expansion of human umbilical cord blood and estimation of its potential for transplantation in adults. Proceedings of the National Academy of Sciences of the United States of America . 1992
  • 3Gluckman E,Broxmeyer HE,Auerbach AD,et al.Hematopoietic reconstitution in a patient with Fanconi’s anemia by means of umbilical cord blood from an HLA-identical sibling. The New England Journal of Medicine . 1989
  • 4Bosma GC,Custer RP,Bosma MJ.A severe combined immunodeficiency mutation in the mouse. Nature . 1983
  • 5Moiser DE,Guliaia RJ,Baird SM,et al.Transfer of a functional human immune system to mice with severe combined immunodeficiency. Nature . 1988
  • 6Lubin I,Faktorowich Y,Lapidot T,et al.Engraftment and development of human T and B cells in mice after bone marrow transplantation. Science . 1991
  • 7Ende N,Giuliani D,Ende M,et al.Production of human to mouse xenografts by umbilical cord blood. Life Sciences . 1990
  • 8Ende N,Ponzio NM,Athwal RS,et al.Murine survival of lethal irradiation with the use of human umbilical cord blood. Life Sciences . 1992
  • 9Meseri A,Delwail V,Lecron JC,et al.NK and LAK cells cytotoxicity for bone marrow and cord blood CFU-GM. Experimental Hematology . 1992
  • 10Broxmeyer HE,Douglas G W,Hangoc G,et al.Human umbilical cord blood as a potential source of transplantable hematopoietic stem progenitor cells. Proceedings of the National Academy of Sciences of the United States of America . 1978

共引文献5

同被引文献14

  • 1Scavadaou A, Isola L, Rubinstein P, et al. A murine model for human cord blood transplantation: near-term fetal and neonatal peripheral blood cells can achieve long-term bone marrow engraftment in sublethally irradiaed adult recipients. Blood, 1997, 89: 1089-1099.
  • 2Levesque JP,Hendy J,Takamatsu Y,et al.Disruption of the CXCR4/CXCL12 chemotactic interaction during hematopoietic stem cell mobilization induced by GCSF or cyclophosphamide.J Clin Invest,2003;111(2):187.
  • 3Yoshikawa M,Tomita Y,Uchida T,et al.Inability of cyclophosphamide-induced tolerance to permit engraftment of pluripotent stem cells contained in a moderate number of syngeneie bone marrow cells.Immunobiology,2000;201(5):552.
  • 4Futami H,Jansen R,Macphee M J,et al.Chemoprotective effects of recombinant human IL-l alpha in cyclophosphamide-treated normal and tumor-bearing mice.J Immunol,1990;145(12):1421.
  • 5Karbicka A,Marchlewicz M,Wiszniewska,B,et al.Bone marrow morphology during haematopoietic stem cell mobilization with cyclophosphamide in mice.Folia Morphol,2003;62(4):435.
  • 6Ramire CD,Sleiman R J,Catchpoole DR,et al.Morphological and molecular evidence of differentiation during etoposide-induced apoptosis.Cell Death Differ,2000;7(8):548.
  • 7Lee JH,Park JH,Yang MH.The effect of cyclophosphamide on fasmediated apoptosis.JKMS,1997;12(2):185.
  • 8项莺松,杨如俊,孟祥顺,蔡建明.小鼠骨髓程序移植的实验研究[J].中华血液学杂志,1998,19(12):634-637. 被引量：26
  • 9童秀珍,洪文德,罗绍凯,龚素珍,彭爱华.鬼臼噻吩甙、高温联用体外诱导白血病细胞HL-60的凋亡及净化[J].中山医科大学学报,2000,21(1):40-42. 被引量：1
  • 10项莺松,杨如俊,唐古生,许熊飞,张明辉,刘书逊,蔡建明,杨平,曹雪涛.不同剂量γ射线照射后胎儿骨髓CD34^+造血干细胞死亡规律[J].中华放射医学与防护杂志,2001,21(2):73-75. 被引量：1

引证文献4

二级引证文献29

临床儿科杂志
1998年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部