木犀草素体内抗炎作用机制研究

Anti-inflammatory mechanism of luteolin in-vivo

目的探讨木犀草素(Lut)的体内抗炎疗效及作用机制。方法采用角叉菜胶致大鼠足爪肿胀模型,观察Lut对足爪肿胀度的影响;酶免疫测定法(EIA)测定炎足足跖部的渗出液前列腺素E2(PGE2)的含量;免疫组织化学法(IHC)及免疫印迹(Western blot)法检测角叉菜胶致炎大鼠足爪组织环氧合酶-2(COX-2)的表达情况。结果Lut可抑制角叉菜胶致炎的大鼠足爪肿胀。80、120 mg/kg Lut可显著降低足爪炎症组织前列腺素E2(PGE2)生成,并显著抑制炎足爪中COX-2的蛋白表达。结论Lut具有较强的抗炎作用,其机制可能与抑制COX-2的表达及PGE2的释放有关。

Objective To investigate the anti-inflammatory effect and mechanism of luteolin in-vivo.Methods The model of carrageenan-induced edema of rat hind paws was prepared.The edema model was treated with luteolin and dexamethasone（for a positive control）.The PGE2 content in the edema exudate was measured by EIA.The COX-2 expression of the edema paws was detected by immunohistochemistry and Western blot.Results Luteolin reduced the paw edema,the PGE2 contents in the exudate and the COX-2 protein expression in the edema paws.Conclusion Luteolin has an anti-inflammatory effect on rat inflammation induced by carrageenan probably through the mechanisms of the inhibition of COX-2 protein expression and of PGE2 production.