PCR和培养法检测泌尿生殖道解脲支原体比较

Comparison of Polymerase Chain Reaction and Culture Method for Assaying Ureaplysma Urealyticum in Urogenital Specimens

目的 对聚合酶链反应(PCR)和培养法检测泌尿生殖道解脲支原体(Uu)进行比较。方法 用上述二种方法同时检测116例患者的泌尿生殖道拭子。结果PCR检测阳性46例,培养阳性45例,培养阳性的45例中PCR阳性42例。若以培养法为对照,PCR敏感性为93.3%,特异性为94.4%。PCR和培养法检测Uu的阳性率无显著差别(P>0.05)。结论 PCR检测泌尿生殖道解脲支原体,敏感性高,特异性强,与培养法比较,前者具有简便、快速的优点。

Objective A comparison study of polymerase chain reaction(PCR) and culture method were used to detect urogenital ureaplasma urealyticum. Methods Urogenital specimens from 116 patients were assayed in parallel by the two methods. Results 46 cases were positive by PCR, 42 cases positive by culture, and UuDNA were dedected in 42 cases of the 45 positive by culture. Compared with culture, the sensitivity and specificity of PCR were 93.3% and 94.4%, respectively. The positive rate of both methods were comparable (P>0.05). Conclusions The PCR technique to detect Uu is highly sensitive and specific. As for culture method, it takes the advantage of being relative simple and takes much less time to give results.