HPLC法测定三七不同药用部位中有效成分含量

Determination of Effective Components in Different Positions of Panax notoginseng by HPLC

目的:建立三七不同药用部位中三七皂苷R_1、人参皂苷Rg_1、Re和Rb_1的HPLC含量测定方法,比较三七不同药用部位中4种皂苷的含量。方法:三七不同药用部位(主根、筋条、剪口、叶、花)甲醇提取,RP-HPLC/UV法测定,Kromasil C_(18)(250 mm×4.6 mm,5μm)色谱柱;以乙腈和水(v/v)梯度洗脱;流速:1 mL/min;柱温:25℃;检测波长:203 nm;进样量:10μL。结果:三七皂苷R_1、人参皂苷Rg_1、Re和Rb_1可达到基线分离;三七皂苷R_1、人参皂苷Rg_1、Re和Rb_1的线性范围分别为:4.4～440μg/mL,4.32～1080μg/mL,4.24～212μg/mL和4.48～1120μg/mL;4种皂苷的精密度和日间差RSD(n=5)分别为0.46%,0.24%,0.77%,0.68%和1.64%,0.69%,0.52%,0.65%;加样回收率(n=5)分别为(102.93±1.22)%,(103.18±0.49)%,(103.20±1.58)%,(103.86±0.39)%;三七不同药用部位含上述4种皂苷总量的顺序为剪口>主根>筋条>花>叶;而三七主根含上述4种皂苷总量的顺序为80头>60头>20头>40头>100头。结论:本方法简便、灵敏、准确、重现性良好,可用于分析三七不同药用部位中上述4种皂苷的含量。

Objective：A quantitative method was developed by gradient elution for the determination of notoginsenoside R1,ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1 in different positions oi Panax Notoginseng by HPLC.The content of 4 kinds saponins in different positions of Panax Notoginseng were compared.Methods：The different positions of Panax notoginseng（including root,rhizome, branch root,leaf,flower） were extracted with methanol.The HPLC condition was as following：Kromasil C18 column（250 mm×4.6 mm,5μm）,acetonitrile and water linearity gradient elution,flow rate at 1.0 mL/min,column temperature at 25℃,wavelength 203 nm.Results：The linear ranges of notoginsenoside R1,ginsenoside Rg1,ginsenoside Re and ginsenoside Rb,were 4.4～440μg/mL, 4.32～1080μg/mL,4.24～212μg/mL and 4.48～1120μg/mL,respectively.The RSD（n=5） of average contents of intra-day and inter-day of 4 kinds saponins were 0.46%,0.24%,0.77%,0.68%and 1.64%,0.69%,0.52%,0.65%.respectively.The average recoveries were（102.93±1.22）%,（103.18±0.49）%,（103.20±1.58）%,（103.86±0.39）%,respectively.The content of 4 kinds saponins in different position of Panax notoginseng was：rhizome〉root〉branch root〉flower〉leaf;the content of 4 kinds saponin in the root of Panax notoginseng was：80 pieces in 500 g〉60 pieces in 500 g〉20 pieces in 500 g〉40 pieces in 500 g〉100 pieces in 500g.Conclusion：This method is simple,sensitive,accurate and repeat,and is suitable in determination of the content of 4 kinds saponins in different positions of Panax notoginseng.