杉叶厥藻多糖的提取、纳米硒化及抗病毒活性研究

Extraction,Selenium-nanoparticle Preparation and Anti-virus Bioactivity Determination of Polysaccharides from Caulerpa taxifolia

目的:从杉叶厥藻中提取多糖类活性物质,并测定其抗病毒活性。方法:水提醇沉法提取粗多糖,以活性为导向,DEAE-Cellulose离子交换层析和Sephadex G-200凝胶柱层析法进行精制。制备的多糖样品测定理化性质,并进行纳米硒化,MTT法测定细胞毒性,CPE法测定抗柯萨奇病毒B3活性。结果:杉叶厥藻粗多糖属硫酸多糖,其多糖平均含量为27.9%,硫酸基平均含量为11.5%;粗多糖纯化得率为66.3%;多糖经纳米硒化后,平均粒径为28.6nm;纯多糖SCp11的半数有效浓度为2.2 mg/mL,治疗指数为3057.0;多糖硒化后,细胞毒性略有增加,但活性增强,治疗指数增加。结论:杉叶厥藻多糖及其硒化产物是一种有开发前景的抗病毒活性物质。

Objective：To extract polysaccharides from Caulerpa taxifolia and determine its anti-virus bioactivity.Methods：The crude polysaccharides were extracted by hot water and precipitated with ethanol,then they were further purified by DEAE-Cellulose and Sephadex G-200 chromatography.After determined by chemicophysical analysis,the polished polysaccharides were selenium treated and bio-assayed by MTT and CPE methods.Results：The crude extraction from Caulerpa taxifolia was a kind of sulfated polysaccharides in which the average content of polysaccharides and sulfate were 27.9%and 11.5%,respectively.The recovery rate of the polished polysaccharides was 66.3%after the 2-column purification.The IC50 and TI of the purified polysaccharide SCp11 were 2.2 mg/ mL and 3057.0,respectively.After treated with selenium,the average diameter of the nanoparticle was 28.6 nm,and its bioactivity and TI index were enhanced significantly although with a higher cytotoxicity.Conclusion：The polysaccharides from Caulerpa taxifolia and its selenium particles was a kind of bioactive substance for anti-virus drug-candidate development.