杜香油β-环糊精包合物的制备及验证

Preparation and Authentication ofβ-Cyclodextrin Complex of Volatile Oil from Ledum palustre

目的：优化β-环糊精（β-CD）包合杜香油的工艺条件。方法：以包合率为指标,以油与β-CD的比例、包合温度、包合时间、β-CD与水的比例为考察因素,采用L9（3^4）正交表设计试验。采用显微成像法、薄层色谱法（TLC）、紫外扫描法（UV）、红外光谱法（IR）对包合物进行验证。结果：杜香油最佳包合工艺为：油与β-CD的比例为1：8,包合温度50℃,包合时间30 min,β-CD与水的比例为1：15。结论：该工艺稳定,质量可控。

Objective：To study the optimum inclusion process conditions for the fl-cyclodextrin complex of volatile oil from Ledum palustre.Methods：The utilization ratio of the volatile oil from Ledum palustre was set as the index,and four factors were selected including the ratio of the volatile oil toβ-cyclodextrin,the temperature of preparation,the inclusion time and the ratio ofβ-cyclodextrin to water.The optimum conditions were investigated by the orthogonal test form L_9（34）.The inclusion compound was identified by the ways of Micro-imaging,Thin-layer chromatography（TLC）,Ultraviolet Spectroscopy（UV） and Infrared Spectroscopy（IR）.Results：The optimum preparation conditions were as follows：volatile oils：β-cyclodextrin was 1：8,the inclusion temperature and time was 50℃and 30 min,respectively,β-cyclodextrin：water was 1：15.Conclusion：The inclusion technology is stable and its quality is adjustable.