膀胱移行细胞癌与突变型P53蛋白表达的研究

Transitional cell carcinoma of bladder and mutant P 53 protein expression

对５１例膀胱移行细胞癌，９例正常膀胱粘膜及８例膀胱慢性炎组织，应用抗Ｐ５３蛋白单克隆抗体进行Ｓ－Ｐ免疫组化染色。结果表明：非癌组织均无Ｐ５３蛋白阳性表达，膀胱移行细胞癌阳性表达率为４９．０２％，且随分化程度的不同，阳性表达率有差异，０～Ⅰ级癌Ｐ５３蛋白阳性表达率为１９．０５％，Ⅱ～Ⅲ级癌Ｐ５３蛋白阳性表达率为７０．００％，两者之间差异有显著性（Ｐ＜０．０５）。此外，有淋巴结转移的移行细胞癌（Ｐ５３癌基因蛋白阳性表达率为６９．５％）与无淋巴结癌转移者（Ｐ５３癌基因蛋白阳性表达率为３２．１４％）两组之间差异有高度显著性（Ｐ＜０．０１）。上述结果提示检测Ｐ５３癌基因蛋白的表达情况可能成为膀胱移行细胞癌分级。

The transitional cell carcinoma of bladder in 51 cases, the normal bladder mucosa in 9 cases, and the chronic cystitis tissue in 8 cases were S-P immunohistochemical stained through anti-monoclonal antibody. Results showed that no positive expression of P 53 protein in non-carcinoma tissue, and the positive rate of 49.02 percent occurred in transitional cell carcinoma of bladder and that is different as variety degree of differentiation, and 19.05 percent positive rate appeared in grade 0～Ⅰ P 53 fusion protein expression and 70.00 percent in grade Ⅱ～Ⅲ, so the difference of the two was significant (P<0.05). Moreover, the difference between the transitional cell carcinoma with lymphatic metastasis group ( 69.5 percent positive rate of P 53 fusion protein ) and the one without lymphatic metasis ( 32.14 percent positiverate of P 53 fusion protein ) is highly significant (P<0.01 ). The mentioned above indicated that P 53 fusion protein expression might be an important reference index for grading transitional cell carcinoma of bladder, estimating the lymphatic metastasis and the prognosis.