从江牛带绦虫感染家猪后血清IL-4和IL-10含量的动态变化

Examination of the serum IL-4 and IL-10 in domestic pigs infecteted with taenia saginata asiatica

目的:观察家猪感染从江牛带绦虫后血清细胞因子IL-4和IL-10含量的变化,从细胞因子角度探讨抗从江牛带绦虫感染免疫应答的机制。方法:用从江牛带绦虫虫卵直接灌胃20 d龄健康乳猪5头,并以健康乳猪5头作对比,于感染后第7、15、45和60天,定期抽取家猪耳缘静脉血,常规分离血清。用ELISA试剂盒检测血清细胞因子IL-4和IL-10含量。结果:正常组血清IL-4和IL-10含量在感染不同时期基本处于稳定水平;实验组血清IL-4和IL-10含量在感染第7天开始上升,第15天最高,第45天含量与正常组相当,第60天再次上升;与正常组相比,实验组血清IL-4和IL-10含量在第7、15、和60天时显著升高(P<0.01)。结论:家猪感染从江牛带绦虫不同时期血清IL-4和IL-10含量变化较大。第7和第15天血清出现高水平IL-4和IL-10,提示体液免疫在抗从江牛带绦虫早期感染中发挥着重要作用;第45天,IL-4和IL-10含量与正常组相比,提示从江牛带绦虫感染家猪中期体液免疫应答变化不大;第60天,高含量的IL-4和IL-10,可能是囊尾蚴诱导肝纤维化形成的重要因素。

Objective： To detect chanism of immune response of domestic pigs against Taenia Saginata asiatica infection through oberserving change of serum IL -4 and IL - 10 in domestic pigs experientially infected with Taenai Saginata asiatica. Methods： Five healthy pigs were fed with eggs of Taenai Saginata asiatica, and reared isolately in animal center, to establish Taenai Saginata asiatica infection model , and normal con- trois. Vein blood of pigs were adopted at day 7, 15, 45and 60 after infection and collected serum. Serum IL - 4 and IL - 10 were detected by ELISA Results： The level of serum IL -4 and IL - 10 of normal group mainmined relatively be stable through out the experiment. The serum IL -4 and IL - 10 level of infection group began to rise at day 7 , the highest at day 15, and increasing again. Compared with the normal group , infected group IL -4 and IL - 10 showed a great increase at day 7 and day 60. Conclusion： Serum IL -4 and IL - 10 level showed biphasic difference among difference infection stage. High level of Sera IL -4 and IL - 10 at ear- ly infected stage suggested that immune response was very important role against Taenia Saginata asiatica at early infected stage. Constant level of IL -4and IL - 10 at day suggested that immune response - associated antigen deceased. IL -4 and IL - 10 might be one important factor in induced liver fibrous in late infection stage.