掺镧氧化钛的制备及其对金黄色葡萄球菌的消毒效能观察

Preparation and observation of disinfection efficacy of inorganic lanthanum-doped titanium compounds on Staphylococcus aureus

目的配制掺镧氧化钛无机制剂,观察其对金黄色葡萄球菌的消毒效能。方法采用溶胶-凝胶法制备出不同温度下掺镧氧化钛6种复合样品,并采用能谱仪进行样品杂质分析。以三角烧瓶振荡试验法测定6种样品中最佳抗菌活性的样品;用试管法测定避光条件下具有最佳抗菌活性的材料对上述细菌的最低抑菌浓度(MIC)与最低杀菌浓度(MBC)值。同时采用电子显微镜观察金黄色葡萄球菌与最佳作用浓度的掺镧氧化钛接触前后超微结构变化。结果通过溶胶-凝胶法获得的6种样品不含任何杂质,其中FH1号样品抗菌活性最强,其对金黄色葡萄球菌ATCC 6538的MIC与MBC值分别为12.5mg/mL、25mg/mL。电子显微镜观察结果提示该材料作用30min,细菌细胞数量明显减少,菌体出现肿胀、固缩或坏死,菌体表面电子密度增高。结论配制的掺镧氧化钛对金黄色葡萄球菌具有良好的杀灭效果。

Objective To prepare inorganic lanthanum-doped titanium （La-TiO2） compounds, and observe the antibacterial activity on Staphylococcus aureus （ATCC 6538）. Methods The six level samples of TiO2 were prepared by sol-gel method. The energy spectrometer technology was used to characterize these products. Antibacterial activity of 6 samples on Staphylococcus aureus were evaluated. Flask Shaking test was used to evaluate the best antibacterial activity among 6 samples; tube test method was used to determine the minimal inhibitory concentration （MIC） and minimal bactericidal concentration （MBC） of sample （code designation was FH1 ） with the best antibacterial activity in dark condition. Change in bacterial ultrastructure was observed by electronmicroscope before and after the action of FH1 for 30 minutes. Results The serials compounds without any foreign materials could be gotten with sol-gel technique, sample 1 （FH1） had the best antibacterial activity. The MIC and MBC of FH1 for Staphylococcus aureus was 12. 5mg/mL and 25mg/mL respectively. The ultrastructure of Staphylococcus aureus changed after exposed to FHI. Conclusion La-TiO2 compounds has good disinfection efficacy on Staphylococcus aureus.