稀土诱导子对红豆杉细胞生长及产生10-去乙酰巴卡亭Ⅲ的影响

The Effect of Rare Earth Chemical Elicitor on the Cell Growth and the Synthesis of 10-Deacetyl Baccatin Ⅲ of Taxus yunnanensis Cell Culture

目的:研究稀土诱导子对云南红豆杉细胞生长及产生10-去乙酰巴卡亭Ⅲ的影响。方法:在培养12d后分别向培养基中添加不同浓度的硝酸铈、硝酸铈铵和硝酸镧诱导子,经过培养对云南红豆杉细胞生长量进行统计,用HPLC法测定细胞中10-去乙酰巴卡亭Ⅲ的含量。结果:培养基中添加0.1mmol/L硝酸铈、0.01mmol/L硝酸铈铵和0.01mmol/L的硝酸镧时,10-去乙酰巴卡亭Ⅲ含量是不添加时的2.3倍、4.1倍和2.6倍,含量分别为0.05446%、0.09767%和0.06187%。结论:该研究为云南红豆杉细胞培养生产10-去乙酰巴卡亭Ⅲ提供理论依据。

Objective： To explore the effects of the rare earth chemical elicitor on the cell growth and the synthesis of 10-deacetyl baccatin Ⅲ. Methods： Different concentrations of chemical elicitors of nitrate cerium, ammonium ceric nitrate and lanthanum nitrate were injected into Taxus yunnanensis cell culture on 12th day, respectively. The concentration of 10-DAB Ⅲ in cell culture was assayed through HPLC analysis; then the cell growth condition of Taxus yunnanensis cell culture was statistically analyzed. Results： The results indicate that it has no significant influence on the Taxus yunnanensis cell culture after the chemical elicitors were injected into culture medium , including nitrate cerium and lanthanum nitrate. It has inhibitory action for the Taxtts yunnanensis cell culture after the high concentration of ammonium eerie nitrate was injected into culture medium. It can promote synthesis and release of 10- DAB Ⅲ markedly when the 0.1 mmol/L nitrate cerium, 0.01 mmol/L ammonium ceric nitrate and 0.01 mmol/L lanthanum nitrate were injected into culture medium respectively, and the content of 10-DAB Ⅲ is 0.05446%, 0.09767% and 0.06187%, 2.3times, 4.1times and 2.6times more than that before injection, Conclusion： The theoretical basis was provided for the fact that the cell culture growth of Taxus yunnanensis could produce 10-DAB Ⅲ.