大理地区牛奶中抗酸阳性细菌的调查

Investigation on Acid-Fast Bacilli of Milk in Dali

目的:探讨大理地区牛奶中抗酸阳性细菌的携带情况,为结核病的防治提供理论依据。方法:选取大理市及周边地区主要奶源供应点,抽取未经消毒处理的新鲜牛奶样本57份,离心后用酸处理,取沉渣涂片,抗酸染色、镜检,同时接种于罗氏培养基上培养,分离抗酸阳性细菌。结果:直接涂片中未见抗酸阳性细菌,罗氏培养基上分离到2株抗酸阳性菌,阳性率为3.5%。结论:大理地区牛奶中检出抗酸阳性细菌,希望有关部门加强牛奶的消毒处理及奶牛结核病的防治。

Objective： To investigate the acid-fast bacilli carrier of milk in Dali and provide a theoretical basis for the prevention and treatment of tuberculosis. Method： 57 unsterilised samples from the urban and rural areas of Dali were collected; after centrifugation and treatment with acid, sediment are smeared for acid-fast stain; at the same time, samples are inoculated on the Lowenstein-Jensen medium for culture, when colonies occur, to observe characteristics of the colonies, perform acid-fast stain and microscopic examination. Result： On the smears, no acid-fast bacterium is observed; two acid-fast bacilli were isolated form Lowenstein-Jensen medium, and the positive rate was 3.5%. Conclusion： Acid-fast bacilli were detected in the milk of Dali, and it＇s necessary to enhance disinfection of milk and surveillance of cattle tuberculosis.