脑外伤患者血清S100β蛋白测定及其临床意义

Determination of Serum S100β Protein in Traumatic Brain Injury Patients and Its Clinical Significance

目的:研究脑外伤患者血清S100β蛋白含量的变化及其临床意义。方法:采用酶联免疫吸附法测定30例脑外伤患者发病后24h内、第3天、第7天及20名健康人的血清S100β蛋白含量,进行动态的检测比较。使用格拉斯哥昏迷计分法(Glasgow ComaScale,GCS)评估疾病状况。以GCS8～3分者为重伤组,共15例。GCS9～15分者为非重伤组,共15例,比较两组在各个时间点的血清S100β蛋白含量有无差异性。结果:两组发病后24h内、第3天、第7天,血清S100β蛋白含量均增高,且于3d达到高峰。重伤组和非重伤组24h内、第3天、第7天、血清S100β蛋白水平与正常对照组相比均明显升高(P<0.05),重伤组明显高于非重伤组。结论:血清S100β蛋白水平可作为反映脑外伤敏感的检测指标,并对患者病情严重程度及预后的评价具有重要的临床意义。

Objective： To research the changes of S100β protein in traumatic brain injury patients and its clinical significance. Method： We used enzyme-linked immunosorbent assay to determine the content of serum S100β protein in 30 traumatic brain injury patients within 24h after onset, 3d, 7d, and 20 healthy people as control group, to analyze serum S100β protein level of patients with traumatic brain injury, with GCS ≤ 8-3 scores for the injured group, a total of 15 cases, GCS ≥ 9-15 scores for non-injured group, a total of 15 eases , to compare differences between the two groups at each time point of serum S100β protein content. Result： In the two groups after injury 24 h, 3d, 7d, the serum S100β protein levels increased and the measurement results vary within 24 hours of severeness and within 24 hours of mildness,（P〈0.05）; Conclusion： The S100β protein level of patients with traumatic brain injury significantly increased, and its severity was positively correlated, and its prognosis was negatively correlated. Serum S100β protein level could serve as a reflection of the extent of traumatic brain injury and the biological indicators of prognosis of the injury