摘要
目的探讨血管内皮生长因子(VEGF)在卵巢子宫内膜异位症组织中表达的意义。方法应用免疫组化技术及体视学方法定量检测30例卵巢子宫内膜异位症(EMs)患者的异位和在位内膜组织中VEGF的平均光密度(MOD)和微血管密度(MVD)的表达,30例非EMs子宫内膜做为对照组。结果VEGF在EMs患者在位内膜中的平均光密度为(0.47±0.12),明显高于EMs患者异位内膜(0.32±0.08)及对照组子宫内膜(0.39±0.14);MVD在EMs患者异位内膜中的表达为(41.58±14.72),明显高于EMs患者在位内膜(31.30±19.89)及对照组子宫内膜(20.70±16.17);VEGF在EMs患者在位内膜和对照组子宫内膜的表达分泌期均高于增生期(P<0.05);VEGF、MVD在EMs组以及对照组中表达均呈正相关,在EMs组在位内膜中的相关性明显高于对照组。结论血管生成是EMs形成的必要条件,在位内膜中高表达的VEGF提示:具有特质性的子宫内膜可能是EMs发病的关键因素。
Objective To investigate the （VEGF） in ovarian endometriosis （EMs） significance of expression of vascular endothelial growth factor Methods Imunohistochemical staining and stereology methods were used to detect the VEGF and MVD in ectopic endometrium of 30 cases, in eutopic endometrium of 30 cases of ovarian endometriosis and normal endometrium in 30 cases. Results The mean optical density of VEGF in eutopic endometrium of endometriosis（0.47 ± 0. 12） was significantly higher than that in ectopic endometrium of endometriosis （0. 32 ± 0.08 ） and the normal control （0.39 ± 0. 14）. The MVD in ectopic endometrium of endometriosis （41.58 ± 14.72） was significantly higher than that in eutopic endometrium of endometriosis （31.30 ± 19. 89） and the normal control （20.70 ± 16. 17）. Theexpressions of VEGF in secretary phase eutopic endometrium of endometriosis and the normal control were significantly higher than that in the proliferative phase （P 〈 0.05 ）. VEGF and MVD had an obviously positive correlation in endometriosis and the normal control, and the positive correlation of VEGF and MVD in eutopic endometrium of endometriosis was higher than that in ectopic endometrium of endometriosis. Conclusion It suggested that there was a prerequisite of neovascularization in the course of the angiogenesis in endometriosis. The high expression of VEGF in eutopic endometrium of endometriosis also suggested that the special quality of the endometrium may be an essential condition in the development of endometriosis.
出处
《中国体视学与图像分析》
2009年第4期333-337,共5页
Chinese Journal of Stereology and Image Analysis
基金
江西省卫生厅科技攻关基金资助项目(20081146)
