安多霖对微波辐射致大鼠脑损伤的防治作用

Preventive and therapeutic effects of a traditional Chinese medicine "AduoLa Fuzhenglin" on the brain injury in rats caused by microwave irradiation

目的探讨安多霖对微波辐射致大鼠脑损伤的防治作用。方法采用30 mW/cm2的微波辐射102只雄性SD大鼠,并于辐射前后给予12 g/kg/d的安多霖每日1次,连续7 d或14 d。在停药后3 d、4 d、5 d、6 d采用Morris水迷宫检测大鼠学习与记忆能力,于辐射后3 d、7 d、14 d和21 d采用光镜和电镜观察脑组织结构的变化,采用图像分析方法对突触间隙宽度、突触后致密物(posts-ynaptic density,PSD)厚度和致密物长度进行定量检测。结果30 mW/cm2微波辐射后大鼠学习记忆能力下降,长期药物治疗组与正常对照组相比无明显差异;30 mW/cm2微波辐射后大脑皮层和海马组织结构和超微结构损伤,表现为神经元固缩,核染色质浓缩、边集,血管周组织间隙增宽,线粒体肿胀、空化,突触间隙不清,穿孔,后膜致密物增厚。药物预防组大鼠脑组织损伤较辐射组为轻,而药物治疗组脑组织损伤明显较辐射组轻。结论30 mW/cm2微波辐射后可损伤大鼠学习记忆能力和脑组织结构,给予12 g/kg/d安多霖对微波辐射所致的上述损伤有一定的保护作用,且治疗性给药保护效果优于预防性给药。

Objective To study the effect of a traditional Chinese medicine ＂ AduoLa Fuzhenglin＂ （ADL） on brain injury in rats induced by microwave irradiation, methods 102 male Wistar rats were irradiated with 30 mW/cm^2 and lavaged by 12 g/kg/d ADL before and after irradiation, once/d, successive administration for 7 d or 14 d. The learning and memory abilities of rats were tested by Morris water maze on day 3,4,5 and 6 after drug withdrawal. The morphological changes of brain tissues were observed on day 3,7, 14 and 21 after drug withdrawal by light and electron microscopy, and the width of synaptic cleft, thickness and length of postsynaptic density were measured by image analysis. Results After 30 mW/cm^2 microwave irradiation, the learning and memory abilities of the rats were impaired. The changes of the drug treatment group were slight, similar to those in the normal group. The structure of brain and hippoeampus were damaged by microwave radiation. The neurons showed ehromatin condensation, the perivascular space was widened, mitochondria swelled and vacuolated, and synaptic clefts were blurred or ruptured, while the postsynaptic density was increased. The injuries of the 30 mW/cm^2 irradiation group were more serious than that of the drug prophylaxis group while the injuries of the latter group was more serious than that of the drug treatment group. Conclusions 30 mW/cm^2 microwave irradiation may impaire the learning and memory abilities and the brain structure in rats. 12 g/kg/d ADL may exert preventive effect on the neuronal injuries induced by microwave radiation and the effect of remedial administration is better than prophylactic administration.