人脑胶质瘤组织中Oct4、Wnt2的表达及其临床意义

Expression of Oct4 and Wnt2 in human glioma tissues and its clinical significance

目的:探讨Oct4和Wnt2在人脑胶质瘤组织中的表达及其与临床病理特征的关系。方法:选取临床及病理资料完整的长江大学附属第一医院2006-2009年手术切除并经病理证实为胶质瘤的石蜡标本56例,采用免疫组织化学方法检测56例胶质瘤组织(其中15例为术后复发)和10例脑外伤行内减压术切除的脑组织标本Oct4、Wnt2的表达。结果:正常脑组织中Oct4表达均为阴性,Wnt2表达仅1例呈弱阳性;56例胶质瘤组织中Oct4阳性34例(60.7%),Wnt2阳性40例(71.4%)。Oct4、Wnt2在低度恶性胶质瘤组中和高度恶性胶质瘤中的阳性率分别为46.2%和73.3%(P<0.05)及57.7%和83.3%(P<0.05),Oct4在复发肿瘤和初诊肿瘤中的阳性率分别为86.7%和51.2%(P<0.05)。人脑胶质瘤组织中Oct4和Wnt2表达呈正相关(r=0.537,P<0.01)。结论:Oct4、Wnt2的表达与人脑胶质瘤的恶性程度相关,Oct4的表达与胶质瘤的复发相关;Oct4可能是通过Wnt通路而参与了胶质瘤的发生、发展。

Objective ：To investigate the expression of Oct4 and Wnt2 in human glioma tissues and its relationship with the clinicopathological features of glioma. Methods： Fifty-six paraffin blocks were obtained from glioma patients receiving surgery. The diagnosis of these patients were confirmed by pathology in our hospital from 2006-2009. Immunohistochemical staining was used to examine Oct4 and Wnt2 expression in the brain tissues of 10 patients with acute brain injury and 56 glioma tissues （including 15 recurrent cases）. Results： The normal brain tissues were negative of Oct4, with only one case showing weak Wnt2 expression. Thirty-four of the 56 glioma tissues showed positive expression of Oct4 （60.7%）, and 40 showed positive expression of Wnt2 （71.4%）. Positive expression rates of Oct4 and Wnt2 in low-grade and highgrade glioma tissues were 46.2 %, 73.3% and 57.7 % , 83.3% , respectively （P 〈 0.05 ）. Oct4 positive rates in the recrudescence and newly diagnosed glioma tissues were 86.7% and 51.2% , respectively （P 〈 0.05 ）. Oct4 expression in the glioma tissues was positively correlated with that of Wnt2 （ r = 0. 537,P 〈 0.01 ）. Conclusion： Expression of Oct4 and Wnt2 is associated with the malignant degrees of glioma, and Oct4 expression is related to the recurrence of glioma. Oct4 might participate in the development and progression of brain glioma through Wnt signaling pathway.