负性协同刺激分子B7-H4在非小细胞肺癌组织中的表达及其临床意义

Expression of negative costimulatroy molecule B7-H4 in non-small cell lung cancer tissues and its clinical significance

目的:探讨负性协同刺激分子B7-H4在原发性非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中的表达及其与NSCLC临床病理特征间的关系。方法:选取南通大学第三附属医院胸外科2008年1月至2009年4月手术切除并经病理证实的NSCLC标本52例。免疫组织化学法检测NSCLC组织中B7-H4分子的表达及CD3+T细胞浸润程度,分析B7-H4表达水平与NSCLC组织中CD3+T细胞浸润、临床病理特征间的关系。结果:52例NSCLC组织中B7-H4表达阳性率为48.08%(25/52),正常肺组织不表达或较少表达B7-H4,差异具有统计学意义(P<0.05)。B7-H4表达水平与NSCLC临床分期和淋巴结转移呈正相关(P<0.05),与CD3+T淋巴细胞浸润程度呈负相关(P<0.05),与其他临床病理参数无关(P>0.05)。结论:负性协同刺激分子B7-H4在NSCLC发病过程中可能起重要的作用,其阳性表达与NSCLC临床分期及淋巴结转移密切相关,B7-H4为NSCLC的诊断及治疗提供参考依据。

Objective： To study the expression of negative costimulatroy molecule B7-H4 in non-small cell lung cancer （NSCLC） tissues and its relationship with the clinical features of NSCLC. Methods ： Fifty-two NSCLC specimens from pa- tients who were pathologically diagnosed in our hospital during January 2008 to April 2009 were included in the present study. B7-H4 expression and infiltration of CD3 ＋T cells in NSCLC tissues were detected by immunohistochemistry. The correlation between B7-H4 expression, CD3＋ T infiltration, and the clinical features of NSCLC was studied. Results ： The positive rate of B7-H4 in 52 NSCLC tissues was 48.08% （25/52） , and B7-H4 expression in normal lung tissues was negative or low （ P 〈 0.05 ）. B7-H4 expression was positively correlated with the clinical tumor stages and lymph node metastasis of NSCLC （ P 〈 0.05 ）, and negatively correlated with tumor infiltration of CD3＋ T cells （ P 〈 0.05 ）, but had no relationship with clinicopathologic parameters of NSCLC （ P 〉 0.05 ）. Conclusion ： Negative costimulatroy molecule B7-H4 may play important roles in the development of NSCLC. Positive expression of BT-H4 is correlated with the clinical tumor stages and lymph node metastasis of NSCLC, which provides a foundation for diagnosis and therapy of NSCLC.