摘要
目的:探讨精子中精核蛋白含量与男性不育的关系、方法:从92例不育男性和16例正常生育男性的精子中提取精核蛋白.在醋酸-尿素聚丙烯酰胺凝胶(acid-ureaPAGE,pH4.7)中电泳.经薄层扫描,测得各蛋白区带(组蛋白和精核蛋白)的相对含量,分析并计算各蛋白区带的相对比值。结果:9例病人的精核蛋白完全缺乏。27例妻子流产男性精子畸形率升高明显(P<0.01)、HP1~3/TBP降低(P<0.01)。精子畸形率与精核蛋白的转化率成负相关。与正常生育组相比,92例男性不育症的总组蛋白(TH)与总碱性蛋白(TBP.包括TH、HP1、HP2、HP3)的比值增高;精核蛋白的含量由正常86.6±5.1%减少为64.1±14.4%(P<0.01);精子密度、精子活动力、畸形率、HP1/TBP、HP2+3/HP1等相差显著(P<0.01)。结论:精核蛋白含量异常的精子与畸形率、活动力、密度以及不明原因的流产等都具有相关性。
Objectives:To study the relation between content of nuclear basic protein and male infertlilty. Methods:Sperm basic nuclear protein was extracted from 92 infertile men and 16 fertile men. The acid soluble protein was eletrophoresed in 12. 5% acid--urea polyacrylamide gels electrophoresis (PAGE ). The relative proportions of histones and protamines were determined by microdensity scanning and ratio of basic nuclear protein was analysed. Results:The results showed that protamine was completely absent in 9 cases. The morphological abnormality of sperm in abortion group was more predominant higher than that in control group (P<0.01 ) while HP1 -- 3/TBP was decreased (P<0.01). The transformation rate of nuclear basic protein was negatively correlated with the morphological abnormality of sperm (r=-0. 79, P<0.01 ). The content of protamine decreased from 86. 6±5. 1% to 64. 1 ±14. 1 %. The quantity and motility, viability teratogenesis of sperm, HP1/TBP, HP2+ 3/HP1 were different significantly. Conclusions: The decrease of protamine is related to sperm teratogenesis,motility,viability and unexplained abortion.
出处
《男科学报》
CSCD
1998年第3期173-177,共5页