摘要
目的检测Snai2 mRNA在正常胃黏膜及胃癌组织中的差异表达并探讨其意义。方法新鲜胃癌组织30例,TRIzol法抽提总RNA,逆转录后Real-time荧光定量PCR检测Snai2 mRNA的表达;制备mRNA探针,在包含有100例胃癌和20例正常胃黏膜组织的芯片上进行Snai2 mRNA原位杂交,并对结果进行统计分析。结果Real-time PCR显示在不同分化的胃癌组织中表达量不同,随分化程度降低,Snai2 mRNA表达量增加(P<0.05);组织芯片原位杂交结果表明Snai2 mRNA在癌组织中的阳性率(54.2%)高于正常胃组织(27.8%),其差异有统计学意义(P<0.05),在不同分化的胃癌组织中Snai2 mRNA的表达也随分化程度的降低,Snai2 mRNA的阳性率逐渐增加,但差异无统计学意义(P>0.05)。结论Snai2可作为一个在分子水平上确定胃癌恶性程度和疾病进展及预后的生物学标志。
Purpose To detect the expression of Snai2 mRNA in gastric carcinoma and normal gastric mucosa and analyze its significance. Methods Total RNA was extracted with TRIzol from 30 cases of gastric carcinoma and mRNA was transcribed reversely into eDNA.The expression of Snai2 mRNA was examined by real-time PCR;then mRNA probes were prepared,the expression of Snai2 mRNA was detected by in situ hybridization on a tissue array,including normal gastric mucosa（20 cases） and gastric carcinoma（100 cases）.Results Real-time PCR data showed they had different expression in different degrees of differentiation of the cancer：the poorer differentiation,the more expression（P〈0.05）.In situ hybridization results showed the positive rate in the carcinoma（54.2%） was higher than normal tissue（27.8%）,with statistically significant difference（P〈0.05）.There were different positive rate in different degrees of differentiation：the poorer differentiation,the higher positive rate,but it had no statistical significance（P〉0.05）. Conclusions Snai2 could be a biomarker of malignant degree and disease progression,and used as a useful tool for evaluation of prognosis.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2009年第6期591-593,597,共4页
Chinese Journal of Clinical and Experimental Pathology
