小鼠肝癌淋巴道转移细胞系中NF-κB基因的表达及意义

Expression of NF-κB gene and its significance in mouse hepatocellular carcinoma cell lines with differently lymphatic metastasis potentials

目的探讨NF-κB基因表达在小鼠肝癌淋巴道转移细胞系中的表达及意义。方法采用RQ-PCR方法检测NF-κB基因在肝癌淋巴道低转移潜能的小鼠肝癌细胞系Hca-P和高转移潜能的小鼠肝癌细胞系Hca-F中的表达水平。结果NF-κB基因在Hca-P和Hca-F细胞系基因表达分别为(1.41±0.48)×10^(-3)、(2.95±0.22)×10^(-3),Hca-P和Hca-F之间,NF-κB基因表达差异有统计学意义(P<0.01),其随转移潜能的增高,表达水平增加。结论NF-κB基因表达水平增高与肝癌淋巴道侵袭转移相关。

Purpose To study NF-κB gene expression level in mouse hepatocellular carcinoma cell lines with differently lymphatic metastasis potentials and to discuss its roles in lymphatic metastasis.Methods Using real-time quantitative PCR,NF-κB gene expression level was detected in mouse hepatocellular carcinoma cell lines,including Hca-P with low lymphatic metastasis potential and Hca-F with high lymphatic metastasis potential.Results NF-κB mRNA expression in Hca-P and Hca-F cell lines were（1.41±0.48）×10^-3,and（2.95±0.22）×10^-3（p〈0.01）,respectively.NF-κB mRNA expression levels were increased with metastasis potential. Conclusion NF-κB gene may play an important role in lymphatic metastasis of hepatocellular carcinoma.