实验性肝纤维化发病过程中脂联素水平变化的研究

Expression of adiponectin in rat with experimental liver fibrosis

目的通过观察肝纤维化大鼠血清及肝组织中脂联素的动态变化,探讨脂联素在肝纤维化形成和发展中的作用。方法将SD大鼠随机分为正常对照组与模型组,腹部皮下分别注射100%橄榄油和40%CCl4-橄榄油溶液,每周2次,共10周,于第2、6、10周末分批取材,肝组织常规石蜡制片,HE染色,光镜下观察,以ELISA法测定大鼠血清脂联素,免疫组织化学法及RT-PCR法检测肝组织脂联素和脂联素mRNA表达。结果模型组大鼠血清脂联素水平随造模时间延长逐步上升,且脂联素及脂联素基因的表达同步增加,各时间点模型组与正常对照组比较差异有统计学意义(P<0.05)。结论脂联素水平的变化可能参与肝纤维化的发病机制。

Objective To discuss the possibility of adiponectin involving the development of liver fibrosis. Methods SD rats were randomly divided into control and model groups which were injected with 100% olive oil and 40% carbon tetrachloride-olive oil solution twice a week for 10 weeks respectively. Samples were obtained at the end of 2,6,and 10 weeks. Liver tissues were analyzed for pathological changes. Serum adiponectin were evaluated by ELISA.The levels of adiponectin protein and adiponectin mRNA were measured with immunohistochemistry and realtime-polymerase chain reaction. Results In the model group,the levels of serum adiponectin gradually increased with the up-regulation of adiponectin protein and adiponectin mRNA expression. Significant differences were observed in adiponectin expression at each time point between the model and control groups（P〈0.05）. Conclusion The variation of adiponectin levels in different stages may be related to the pathogenesis of liver fibrosis.