局部湿热联合微波消融对小鼠乳腺癌动物模型中树突状细胞的比例和功能的影响

Effects of local wet hyperthermia combined with microwave ablation on the proportion and function of dendritic cells in animal model of mouse mammary carcinoma

目的:研究局部湿热联合微波消融对乳腺癌荷瘤小鼠脾细胞中树突状细胞的比例和功能的影响,并探讨其意义.方法:建立小鼠乳腺癌实验动物模型;用FACS检测局部湿热联合微波消融处理后荷瘤小鼠脾细胞中DC细胞比例变化及其MHC-Ⅱ类分子和协同刺激分子CD80,CD86的表达水平;MTT法检测DC细胞对T淋巴细胞增殖的刺激作用;FACS检测DC细胞对T细胞的细胞因子IFN-γ,IL-4分泌的影响.结果:局部湿热联合微波消融处理组荷瘤小鼠脾细胞中DC细胞的比例明显增高,DC细胞的MHC-II类分子和协同刺激分子CD86的表达明显上调,并且DCs刺激T淋巴细胞增殖和IFN-γ分泌的能力明显增强.结论:局部湿热联合微波消融术可以较单一热疗方法更有效的增加DC细胞的比例和增强其功能.

AIM：To investigate the effects of local wet hyperthermia combined with microwave ablation on the proportion and function of dendritic cells（DCs） in animal model of mouse mammary carcinoma and to explore its significance.METHODS：An experimental animal model of mouse mammary carcinoma was established and then treated with local wet hyperthermia combined with microwave ablation.The proportion of DCs in splenocytes from tumor bearing mice was firstly detected by FACS.The expression of MHC class II molecule,as well as co-stimulatory molecule CD80 and CD86,on DCs were also analyzed by FACS.The proliferation of T cells stimulated by DCs was determined by MTT assay.Moreover,the IFN-γ and IL-4 production of T cells induced by DCs were also analyzed by FACS.RESULTS：The proportion of DCs and the expression level of MHC class II molecule and CD86 on DCs in local wet hyperthermia combined with microwave treated group were significantly higher than those of microwave ablation or local wet hyperthermia treated alone group（P0.05）.Furthermore,compared with control groups,both the proliferation and IFN-γ production of T cells stimulated by DCs increased significantly in local wet hyperthermia combined with microwave treated group.CONCLUSION：Local wet hyperthermia combined with microwave ablation more effectively increases the proportion and function of DCs than single local wet hyperthermia or microwave ablation.