小鼠胚胎成纤维细胞介导的IL-12抗S-180肿瘤作用

Anti-tumor effects of mouse embryonic fibroblasts-mediated interleukin-12(IL-12) on Sarcoma 180 xenografts

目的:将IL-12重组载体转染入小鼠成纤维细胞,研究探讨转染后的抗肿瘤作用.方法:取小鼠胚胎获得原代成纤维细胞,应用PEI法将IL-12重组载体转染到小鼠胚胎成纤维细胞,同时设对照.建立肉瘤细胞(S-180)荷瘤小鼠模型,分别接种S-180细胞.于注射瘤细胞后定期给予转染后的成纤维细胞瘤体内注射,并设对照组.于致瘤后第21日处死小鼠,测肿瘤质量;ELISA法检测小鼠血清IL-12及γ-干扰素(IFN-γ)的表达;检测NK(natural killer cells)细胞活性和脾淋巴细胞增殖情况、CD4/IFN-γ、CD8/IFN-γ双标记阳性细胞的克隆能力.结果:抑瘤率、NK细胞活性及淋巴细胞增生反应、血清IL-12、IFN-γ水平、CD4/IFN-γ及CD8/IFN-γ双标记阳性细胞的百分率均高于对照组(P<0.01).结论:转染后的IL-12重组质粒可进一步增强机体的免疫功能发挥其抗肿瘤的治疗作用.

AIM ： To investigate the anti-tumor effects of mouse embryonic fibroblasts-mediated interleukin-12（IL-12） on Sarcoma 180 xenografts. METHODS： The murine normal fibroblast were transfected with human IL-12 gene（FB/IL-12） by PEI. Controls were set up at the same time. The S-180 ceils were inoculated in mice subcutaneously. Then FB/IL-12 was injected in micc in the same place subcutaneously at intervals, and controls were set up. On the 21st day, tumor weight was recorded. The serum levels of IL-12 and the expression of interferon-γ （IFN-γ） in mice were detectedby EILISA. The activity of the natural killer cells （NK） and spleen lymphocyte were detected; the percentages of CD4^＋ /IFN-γand CD8 ＋/IFN-γ double positive cells were also investiga- ted. RESULTS： The proliferation inhibition rate of FB/IL-12 group was higher than those of the controls （ P 〈 0.01 ）. The activity of NK cells and proliferation of Lymphocytes, the serum levels of IL-12 and IFN-γ, the frequency of CD4＋/IFN-γ and CD8 ＋/ IFN-γ double positive cells were also higher than controls （P 〈 0.01 ）. CONCLUSION： By intensifying its immune ability, the mouse embryonic fibroblasts-mediated interleukin-12 （IL-12） on Sarcoma 180 xenografts has better anti-tumor effects.