SOCS3基因沉默对高脂餐大鼠脂质代谢紊乱的影响

Effects of NRAi-mediated gene silencing of SOCS3 on metabolic disturbance in high-fat diet rats

目的:探讨通过RNA干扰技术下调SOCS3表达防治高脂餐大鼠代谢紊乱的作用.方法:取SD6wk龄大鼠45只,随机分为3组:普通饮食对照组(n=15),下丘脑注射空白质粒,普通饲料喂养;高脂饮食对照组(n=15),下丘脑注射空白质粒,高脂饲料喂养;实验组(n=15),下丘脑注射shR-NA-SOCS3序列慢病毒重组体,高脂饲料喂养.每周测体质量1次.饲养8wk末,测空腹血糖和体质量,处死后立即取心脏血进行血清胰岛素、三酰甘油、总胆固醇、血清瘦素测定.结果:实验组体质量、三酰甘油、总胆固醇低于高脂饮食对照组(P<0.01),高于普通饮食对照组(P<0.05).实验组血清瘦素、血清胰岛素浓度低于高脂饮食对照组组(P<0.01),与普通饮食对照组比较无统计学差异.结论:下调SOCS3基因表达可以降低体质量、三酰甘油、血清胰岛素、总胆固醇及血清瘦素水平,对肥胖症、糖尿病及慢性并发症可能有预防和治疗作用.

AIM：To study the effects of NRAi-mediated gene silencing of SOCS3 on metabolic disturbance in high-fat diet rats.METHODS：Forty five 6-week-old healthy Sprague-Dawley（SD） rats were adopted to our research and divided into 3 groups randomly.In the standard control group（n=15）,the control lenti were stereotaxically injected into the hypothalamus of rats which then fed with standard chow;In the high-fat diet control group（n=15）,the control lenti were stereotaxically injected into the hypothalamus of rats which then fed with high-fat diet;In experiment group（n=15）,the SOCS3 lenti were stereotaxically injected into the hypothalamus of rats which then fed with high-fat diet.Animal weight was measured weekly.Up to 8 weeks after treatment,before exeduted,fasting plasma glucose was measured,After exeduted,serum was collected for measurement of serum leptin,insulin,triglyceride,total cholesterol.RESULTS：The experiment group rats gained smaller weight,lower triglyceride and total cholesterol than high-fat control group rats（P0.01）and showed larger weight,higher triglyceride and total cholesterol than CS rats（P0.05）.Levels of serum leptin and insulin were lower in experiment group rats than high-fat control group rats（P0.01）.There were no significant differences in comparison of levels serum leptin and insulin in experiment group rats with the standard control group rats.CONCLUSION：Body weight,serum leptin,insulin,triglyceride and total cholesterol concentration were decreased through down-regulation of SOCS3 expression by RNAi-mediated gene silencing of SOCS3,suggesting that down-regulation of SOCS3 expression is a promising therapeutic approach in obesity,diabetes and chronic complications.