乌拉坦增强延长离体大鼠心脏的低温保存效果

Myocardial protective effects of urethane on isolated rat heart in prolonged hypothermic preservation

目的:观察乌拉坦对延长离体大鼠心脏的低温保存作用.方法:成年雄性SD大鼠分为两组(n=6):对照组:HTK保存液;试验组:HTK保存液加乌拉坦(30mmol/L).心脏分别置入两种保存液4℃下保存6和18h.采用Langendorff心脏灌注装置进行保存前及保存后再灌注30min时心脏功能测定,比较心脏功能恢复率、ATP含量、细胞凋亡和超微结构变化.结果:低温保存后实验组左室功能恢复率较对照组明显升高,细胞凋亡及超微结构损伤较对照组减轻;保存18h后实验组ATP含量显著高于对照组.结论:乌拉坦加入HTK心脏保存液能够显著增强离体大鼠心脏的低温保存效果.

AIM：To observe the protective effects of urethane plus HTK on the isolated rat hearts in prolonged hypothermic preservation.METHODS：Male Sprague-Dawley rats（250-280 g） were divided into 2 groups（n=6 per group） according to each preservation solution used：control group,HTK solution;experimental group,HTK solution plus urethane.The hearts were arrested and stored in each preservation solutions respectively for 6 and 18 h at 4℃.After preservation,the myocardial function,ATP content,cardiomyocyte apoptosis and ultrastructure were examined.RESULTS：Compared with control group,experimental group showed higher recovery of cardiac function,lower rate of cardiomyocyte apoptosis and better cardiomyocyte ultrastructure both for 6 and 18 h of preservation.The ATP content after 18 h of preservation in control group was significantly higher than that in experimental group.CONCLUSION：Urethane led to significantly better and longer preservation in isolated hearts subjected to prolonged hypothermic preservation.