CD44^+/CD24^-表型在乳腺癌分子亚型中的表达和分布

Expression and disposition of CD44^+/CD24^-phenotype in molecular subtypes of breast cancer

目的:研究CD44+/CD24-表型在乳腺癌中的表达并探讨其与乳腺癌各分子亚型的相关性.方法:根据免疫学标志物(ER,PR,HER2,CK5/6,P53)把217例乳腺癌划分为5类分子亚型:basal-like型、LuminalA型、LuminalB型、Her2过表达型和Normalbreast-like型.应用双重免疫组织化学检测CD44/CD24双染的情况,分析CD44+/CD24-表型与乳腺癌5个分子亚型的相关性.结果:217例乳腺癌病例中,luminalA型130例、luminalB型15例、HER2过表达型21例、basal-like型29例、Normalbreast-like型22例.38%的乳腺癌组织表达CD44+/CD24-表型,CD44+/CD24-表型在basal-like型、Lu-minalA型、LuminalB型、Her2过表达型、Normalbreast-like型乳腺癌中表达率分别为69.0%,36.2%,26.7%,19.0%,31.8%.CD44+/CD24-表型在乳腺癌各分子亚型中的表达具有显著差异性(P=0.003),高表达在basal-like乳腺癌中(69%).结论:CD44+/CD24-乳腺癌干细胞与basal-like乳腺癌有相关性;其他乳腺癌干细胞标志物仍需得到进一步鉴定.

AIM：To study the expression of CD44＋/CD24-phenotype and its association with the molecular subtypes of breast cancer in breast cancer.METHODS：Using immunostaining ER,PR,HER2,CK5/6 and P53 to classify the material into 5 molecular subgroups：basal-like subgroup,Luminal A subgroup,Luminal B subgroup,Her2 over-expressing subgroup and Normal breast-like subgroup.Double-staining immunohistochemistry（IHC） was applied for the detection of CD44＋/CD24-cells.The correlations of the expression of the CD44＋/CD24-to molecular subtypes of breast cancer were analyzed.RESULTS：In 217 cases of breast cancer,basal-like subgroup：29 cases,Luminal A subgroup：130 cases,Luminal B subgroup：15 cases,Her2 over-expressing subgroup：21 cases,Normal breast-like subgroup：22 cases.The expression rates of CD44＋/CD24-in total breast cancer,basal-like subgroup,Luminal A subgroup,Luminal B subgroup,Her2 over-expressing subgroup,Normal breast-like subgroup were 38%,69%,36.2%,26.7%,19%,31.8%.The expression rates of CD44＋/CD24-phenotype was high in basal-like breast cancer,which was significantly different from the other subgroups（P=0.003）.CONCLUSION：The study suggests that the CD44＋/CD24-breast cancer stem cells in tumor tissue is associated with basal-like cancer,and other breast cancer stem cell markers remain to be identified.