河南食管癌高发区同一样本新鲜和石蜡固定组织人乳头瘤病毒检测差异分析

Comparative analysis on human papillomavirus infection with handing of different methods in high incidence area for esophageal cancer in Henan

目的:探讨人乳头瘤病毒(HPV)在高发区食管癌中差异性检测的原因.方法:利用聚合酶链式反应(PCR),对同一食管癌手术切除标本,从新鲜组织和石蜡固定组织两方面进行HPV16-DNA检测.结果:30例食管癌新鲜组织HPV16感染率为84%,对应石蜡固定组织为53%;新鲜组织中3例HPV16阴性的标本在其对应的石蜡固定组织中3例均为阴性,27例HPV16阳性的新鲜组织标本中其对应石蜡固定组织中仅16例检测到HPV16-DNA.结论:HPV16-DNA在食管癌中的差异性检测结果与材料处理方式不同有关,尤其是固定组织在处理过程中,DNA断裂,形成一些小分子片段,以至于超出所要扩增的范围.

AIM： To explore the reasons of human papillomavirus （HPV） in esophageal cancer detected vary. METHODS： HPV16-DNA detected by polymerase chain reaction（PCR） in 30 the same surgical resection of esophageal cancer specimens in fro- zen and paraffin embedded matched. RESULTS： Detection rates of HPV involved in 30 frozen esophageal cancer specimens was 84% ,53% in corresponding to paraffin fixed tissue, respectively. Three frozen specimens with HPV-negative in their paraffin coun- terparts were negative, twenty-seven cases frozen tissue samples with only 16 cases in paraffin detected HPV16-DNA in existence. CONCLUSION ： For the highly divergent detection rates of HPV in esophageal carcinoma, in addition to different geographical factor, by the application of different detection methods, it should be attributed to materials processed in different methods, and caused a lot of small DNA fragmentation, even beyond the scope to be amplified.