摘要
目的研究半乳糖凝集素-3(Galectin-3,Gal-3)对胚胎大鼠脊髓神经干细胞(neural progenitor/stem cells,NPCs)的增殖、凋亡及向神经元分化的影响。方法取孕14 d的胚胎大鼠脊髓,原代培养出脊髓NPCs,传代3次后分别加入不同浓度的Gal-3,采用WST-8法和Hoechst 33258染色检测NPCs的增殖活力和凋亡情况,抗β-tubulinⅢ(Tuj1)细胞免疫荧光染色检测神经元分化比例,并作统计学分析。结果与对照组相比,3种不同浓度的Gal-3(1μg/mL、4μg/mL及8μg/mL)对NPCs增殖活力和凋亡的影响差异均无统计学意义(P>0.05);然而3种不同浓度的Gal-3均能促使NPCs向神经元分化的比例增加(P(0.01),对照组与实验组的神经元分化比例分别为7.23%±1.07%、15.10%±1.56%、14.57%±2.01%和15.30%±2.30%,不同剂量组间比较差异均无统计学意义(P>0.05)。结论不同浓度的Gal-3对NPCs的增殖和凋亡均无明显影响,但都有促进NPCs向神经元方向分化的作用。
Objective To investigate the effects of galectin-3 on the growth, apoptosis and differentiation into neurons of embryonic rats spinal neural progenitor/stern cells （NPCs） in vitro. Methods NPCs were obtained from Wistar rats at embryonic day 14 and cuhured in serum-free medium. After 3 passages, the effects of Gal-3 on the proliferation activity and apoptosis of NPCs were determined by WST-8 assay and Hoechst 33258 stain when different concentrations Gal-3 were applied. Anti 13-tubulin Ⅲ （Tujl） immunostaining was used to label the neurons differentiated from NPCs, and the percentage of Tujl positive cells among Hoechst positive cells. Statistical analysis was also conducted. Results Compared with the control group, no statistical difference was observed on the proliferation activity and apoptosis of NPCs under the presence of different concentration （1 μg/mL, 4 μg/mL and 8 μg/mL） of Gal-3 （P 〉 0.05 ）. However, Gal-3 could induce the differen- tiation of NPCs into neuron （P 〈 0.01 ） , and the proportion of neurons in the control group and the experimental groups was 7.23% ± 1.07% , 15.10% ± 1.56% , 14.57% ±2.01% and 15.30% ±2.30% , respectively. But there were no statistical difference among the 3 concentrations above （ P 〉 0. 05 ）. Conclusion Different concentrations of Gal-3 can not affect the proliferation and apoptosis of NPCs, but have the effect of promoting the differentiation of NPCs into neurons.
出处
《脊柱外科杂志》
2009年第6期361-364,共4页
Journal of Spinal Surgery
基金
国家自然科学基金资助项目(30572165)
