内皮化环氧交联牛心包材料动物体内抗钙化的研究

The Anticalcification Effect of Endothelialization of Bovine Pericardium Cross Linked with Polyepoxy Compound

目的研究生物材料内皮化延缓钙化的效果，从材料学上改进生物瓣性能，以提高其耐久性。方法将环氧交联的牛心包材料体外内皮化后同时进行犬股动脉间位移植和腹部皮下包埋，与单纯戊二醛处理者和单纯环氧处理者进行钙化程度对比。结果钙含量测试结果以内皮化环氧交联材料皮下包埋最低，未内皮化材料中以环氧处理者钙含量较低。结论环氧交联牛心包材料可实现体外快速内皮化，生物材料的内皮化“活化”有赖于表面全部被自体存活内皮细胞覆盖才能实现，完全的内皮化确有抗钙化作用。

Objective The possibility of improving the performance of bioprosthetic valve by means of preendothelialization with cultured autologous cells has been suggested. The aim of this study is to investigate the availability of endothelialization on polyepoxy compound (PC) cross linked bovine pericardium and then certify the anti calcificational effect of endothelialization. Methods The cultured dog saphenous vein endothial cell (EC) were seeded onto PC cross linked bovine pericardia, then the pericardia were transplanted in femoral arteries and subcutis of the same dog from which EC had been cultured. Unendothelialized PC or glutaraldehyde (GA) cross linked pericardia were transplanted as control. Results For subcutis groups, endothelialization reduced the diffusion of calcium significantly, however, the same result did not appear for artery groups. Conclusion Endothelialization of polyepoxy compound cross linked bovine pericardium is available. Complete endothelialization can significantly inhibit calcification of the bioprostheses.