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茉莉酸甲酯诱导人肝癌细胞HepG2凋亡作用机制的实验研究 被引量：5

MeJ A Induces Hepatocarcinoma HepG2 Cells Apoptosis and its Mechanism
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摘要 目的探讨茉莉酸甲酯诱导人肝癌细胞HepG2发生凋亡的作用机制.方法琼脂糖凝胶电泳检测HepG2细胞凋亡,RT-PCR检测HepG2细胞中Bcl-2、BaxmRNA表达,免疫细胞化学检测HepG2细胞Bcl-2、Bax蛋白表达.结果MeJA作用HepG2细胞48h后:DNA琼脂糖凝胶电泳可见明显典型"梯"状条带;细胞Bcl-2mRNA表达水平明显下降(P<0.05)而BaxmRNA表达水平明显增高(P<0.05);Bcl-2蛋白在胞浆中表达水平明显降低(P<0.01)而Bax蛋白表达水平显著增加(P<0.01).结论茉莉酸甲酯通过降低抑凋亡相关基因Bcl-2mRNA及蛋白的表达,上调促凋亡相关基因BaxmRNA及蛋白的表达,从而诱导HepG2细胞凋亡的发生. Objective To study the mechanism of MeJA induced HepG2 cells apoptosis.Methods We detected cell apoptosis by AGE,used RT-PCR to detect the mRNA expression of Bcl-2,Bax in HepG2 cells,and detected protein expression of Bcl-2,Bax in HepG2 cells by immunocytochemistry.Results After MeJA acting on HepG2 cells for 48 h：Obviously typical ladder straps was found in DNA AGE;the mRNA expression of Bcl-2 decreased（P〈0.05） while the mRNA expression of Bax obviously increased（P〈0.05）.the protein expression of Bcl-2 in endochylema is obviously lower than the expression in control group（P〈0.01）;while the protein expression of Bax increased compared with control group（P〈0.01）.Conclusions MeJA can induce HepG2 cells apoptosis by decreasing the mRNA and protein expression of apoptosis-depressing gene Bcl-2,and in creasing the mRNA and protein expression of apoptosis-promoting gene Bax.
作者 卢永刚 张洁 范吉利 谭晶 郭峰 郭永章
机构地区 柳铁中心医院普外科 昆明医学院
出处 《昆明医学院学报》 2009年第12期33-37,共5页 Journal of Kunming Medical College
关键词 茉莉酸甲酯 全反式维甲酸 HepG人肝癌细胞 凋亡相关基因 琼脂糖凝胶电泳 表达水平 蛋白表达 作用机制 对照组 表达差异 Methyl jasmonate All-trans-retinoic acid HepG2 human hepatocellular carcinoma cell Apoptosis
分类号 R657.52 [医药卫生—外科学]
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参考文献13

  • 1卢永刚,张洁,谭晶,郭峰,郭永章.茉莉酸甲酯抑制人肝癌细胞HepG2增殖作用的实验研究[J].昆明医学院学报,2008,29(5):75-81. 被引量：7
  • 2PENG L,LIU J J. A novel method for quantitative analy - sis of apoptosis [ J ]. Lab Invest, 1997,77 (6) : 547 - 555.
  • 3WALKER P R, SIKORSKA M. New aspects of the mec - hanism of DNA fragmentation in apoptosis [J]. Biochem Cell Biol, 1997,75(4) :287 - 299.
  • 4CONRADT B,XUE D. Programmed cell death[J]. Worm Book, 2005,6 : 1 - 13.
  • 5TOPCHI N N,BERDYSHEV G D,TISHCHENKO E N, et al. Programmed death of the eukaryote cells [J]. Aviakosm Ekolog Med, 2004,38(6) : 3 - 15.
  • 6YOULE R J, STRASSER A. The BCL-2 protein family:opposing activities that mediate cell death [J]. Nat Rev Mol Cell Biol,2008,9( 1 ) :47 - 59.
  • 7VERDINE G L, WALENSKY L D. The challenge of drugging undruggable targets in cancer:lessons learned from targeting BCL-2 family members [J]. Clin Cancer Res, 2007,13 (24) : 7264 - 7270.
  • 8ZIMMERMANN K C ,BONZON C ,GREEN D R. The machinery of programmed cell death [J]. Pharmacol Ther, 2001,92( 1 ) : 57 - 70.
  • 9HEIMLICH G, MCKINNON A D, BERNARDO K, et al. Bax-induced cytochrome c release from mitochondria depends on alpha-helices-5 and -6 [J]. Biochem J, 2004,378(Pt 1 ) :247 - 255.
  • 10LANAVE C,SANTAMARIA M,SACCONE C. Comparative genomics: the evolutionary history of the Bcl-2 family [J]. Gene,2004,333:71 - 79.

二级参考文献13

  • 1KERR J F R. Apoptosis:a basic biological phenomenon w ith wide Ranging implications in tissue kinetics [J]. Br J Cancer, 1972,26(4) : 239.
  • 2ELIEZER, FLESCHER. Jasmonate-a new family of anti-cancer agents [J]. Anti-cancer Drugs,2005,16(9): 911 -916.
  • 3FINGRUT O , FLESCHER E. Plant stress hormones suppress the proliferation and induce apoptosis in hu man cancer cells[J]. Leukemia,2002,16(4):608 - 616.
  • 4KIM J H, LEE S Y, OH S Y,et al. Methyl jasmonate induces apoptosis through induction of Bax/Bcl-XS and activation of caspase-3 via ROS production in A549 cells [J]. Oncol Rep,2004,12(6): 1233 - 1238.
  • 5ADACHI Y,ITOH F,YAMAMOTO H,et al. Retinoic acids reduce matrilysin (matrix metalloproteinase 7)and inhibit tumor cell invasion in human colon cancer [J]. Tumor Biol, 2001,22(4) : 247 - 253.
  • 6KLAASSEN I, BRAKCNHOFF R H, SMEETS S J, et al. Expression of retinoic acid receptor gamma correlates with retinoic acid sensitivity and metabolism in head and neck squamous cell carcinoma cell lines [J]. Int J Cancer, 2001,92(5) :661 - 665.
  • 7ALOE K, DOSAKA A H, MURAKAMI A, et al. A combination treatment of c-myc antisense DNA with all-trans-retinoic acid inhibits cell proliferation by downregulating c-myc expression in small cell lung cancer. Antisense nucleic acid drug[J]. Dev,2000,10 (4) : 243 - 249.
  • 8CHOI S H,KANG H K,IM E O,et al. Inhibition of cell growth and telomerase activity of breast cancer cell in vitro by retinoic acids [J]. Int J Oncol,2000,17 (5):971 - 976.
  • 9PENDINO F, FLEXOR M, DELHOMMEAU F, et al. Retinoids downregulate telomerase and telomere length in a pathway distinct from leukemia cell differentiation [J]. Proc Natl Acad Sci USA ,2001,98(12) :6662 - 6667.
  • 10HSU S L,CHENG C C,SHI Y R,et al. Proteolysis of integrin alpha 5 and beta 1 subunits involved in retinoie acid-induced apoptosis in human hepatoma Hep3B cells [ J ]. Cancer Lett, 2001,167 (2) : 193 - 204.

共引文献6

同被引文献36

  • 1董俊峰　,吴小鹏.原发性肝细胞癌Survivin,CyclinD1的表达及其临床意义[J].中国现代普通外科进展,2007,10(2):156-159. 被引量：9
  • 2PRUDHOMME M.Novel checkpoint 1 inhibitors[J].Recent Patents Anticancer Drug Discov,2006,1(1):55-68.
  • 3DASS,BOSWELLS A,AARONSON SA,LEES W.P53 promoter selection:choosing between life and death[J].Cell Cycle,2008,7(2):154-157.
  • 4WANG W,El-DEIRY W S.Restoration of p53 to limit tumor growth[J].Curt Opin Oncol,2008,20(1):90-96.
  • 5SONG H,XU Y.Gain of function of p53 cancer mutants in disrupting critical DNA damage response pathways Cell Cycle,2007,6(13):1570-1573.
  • 6DEY A,VERMA C S,LANE D P.Updates on p53:modulation of p53 degradation as a therapeutic approach[J].Br J Cancer,2008,98(1):4-8.
  • 7WOLF D,HARRIS N,ROTTER V.Reconstitution of p53 expression in a nonproducer Ah-MuLV-transformed cell line by transfection of a functional p53 gene[J].Cell,1984,38(1):119-126.
  • 8GUILLOTC,FALETTE N,PAPERIN M P,et al.p21(WAF1/CIP1)response to genotoxie agents in wild-type TP53 expressing breast primary tumours[J].Oncogene,1997,14(1):45-52.
  • 9FUJIWARA K,DAIDO S,YAMAMOTO A,et al.Pivotal role of the cyclin-dependent kinase inhibitorp21WAF1/CIP1 in apoptosis and autophagy[J].J Biol Chem,2008,283(1):388-397.
  • 10PAUNESKU T,MITTAL S,PROTI M,et al.Proliferatingcell nuclear antigen (PCNA):ringmaster of the genome[J].Int J Radiat Biol,2001,77(10):1007-1021.

引证文献5

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昆明医学院学报
2009年 第12期

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