大鼠异位心脏移植模型的建立

A Effective Heterotopic Heart Transplantation Model in Rat

目的建立一种快速、有效的大鼠异位心脏移植模型.方法以雄性SD大鼠为供体,Wistar大鼠为受体,将供心的升主动脉与受体的腹主动脉行端侧吻合,切除受体的左肾,将供心的肺动脉与受体的左肾静脉以自制聚乙烯管直接插入法连接.结果35例实验成功32例,成功率91.4%,供心平均缺血时间为(37.8±1.8)min.失败的原因:吻合口出血,血栓形成.结论本术式操作简单实用,不需手术显微镜,易于推广.

Objective To establish a quick and effective heterotopic heart transplantation model in rat.Method Male SD rats served as donors and Wistar as recipients,the ascending aorta of allograft was anastomosed to the recipient abdominal aorta,The recipient underwent a left nephrectomy,and the right pulmonary artery of allograft inserted into the left renal vein of donors.Results The mean ischemic time was（37.8 ± 1.8） min in 35 experiments.The successful rate was 91.4%.Complications were anastomotic orificces hemorrhange,thrombosis.Conclusion This method has advantage of simpleness,and requiring no operating microscope.So it is easy to be spreaded.