大肠癌患者与正常人肠道菌群的比较研究

The Comparative Research on Intestinal Flora Population of Patients with Colorectal Cancer and Normal People

目的比较大肠癌患者与正常人的肠道菌群不同点,为大肠癌的防治提供新的思路.方法大肠癌(包括结、直肠癌)患者镜检取材、病理证实患者30例,正常对照组人群30例,所选病例为正常人群、大肠癌患者手术前未做肠道准备,晨起排便.稀释性平板菌落计数法+快速细菌鉴定法:取材,定量,稀释,37℃分别做厌氧、需氧培养,涂片镜检,然后分别对各菌株进行鉴定并按平板活菌菌落计数法计数.结果正常对照组与实验组比较结果:大肠癌患者组大肠杆菌、粪肠球菌计数显著增加;双歧杆菌,乳酸杆菌、类杆菌(弯曲杆菌)计数显著减少.结论大肠癌患者存在大肠杆菌、粪肠球菌数量增加;双歧杆菌,乳酸杆菌、类杆菌(弯曲杆菌)数量减少.加强对大肠癌患者肠道菌群数量改变(失调)的研究,并采取相应的对策、措施,可能对大肠癌的防治具有重要的意义.

Objective To compare intestinal tract florae between colorectal cancer patient and normal people,in order to offer new clue for prevention and cure of colorectal cancer.Methods Colorectal cancer in patients with microscopic examination subjects and pathologically was confirmed in 30 cases,compared with 30 cases of normal control group population;the selected cases for the normal population,patients with colorectal cancer without making bowel preparation before surgery were defecated in the morning.Diluted plate colony count method＋rapid bacteria identification method：Quantitative,dilution,37℃ separately anaerobic or aerobic cultures,smear microscopy,and then the bacterial strains count and species identification were carried out with the method of plate counting live bacterium.Results The normal control group was compared with the results of patients with colorectal cancer：for patients with colorectal cancer,E.coli and fecal enterococci counts increased significantly;Bifidobacterium,Lactobacillus,Bacteroides（Campylobacte）r counts decreased significantly.Conclusions For patients with colorectal cancer,E.coli and Enterococcus faecalis increase in numbers;Correspondingly,Bifidobacterium,Lactobacillus,and Bacteroides（Campylobacte）r counts decrease significantly.Enhancing the study of intestinal flora in patients with colorectal cancer and continuously taking the appropriate measures in prevention and treatment of colorectal cancer may have important significance.